Anti-Fibronectin antibody (ab23751)
Key features and details
- Rabbit polyclonal to Fibronectin
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Fibronectin antibody
See all Fibronectin primary antibodies -
Description
Rabbit polyclonal to Fibronectin -
Host species
Rabbit -
Specificity
Fibronectin 100%, collagen type I, III and IV <0.1%, elastin <0.1% -
Species reactivity
Reacts with: Rat, Human, Pig -
Immunogen
Full length native protein (purified) corresponding to Rat Fibronectin.
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General notes
This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Primary antibody notes
This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Target
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Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. -
Tissue specificity
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. -
Involvement in disease
Glomerulopathy with fibronectin deposits 2 -
Sequence similarities
Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. -
Developmental stage
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. -
Post-translational
modificationsSulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 2335 Human
- Entrez Gene: 397620 Pig
- Entrez Gene: 25661 Rat
- Omim: 135600 Human
- SwissProt: P02751 Human
- SwissProt: Q9GLP0 Pig
- SwissProt: P04937 Rat
- Unigene: 203717 Human
see all -
Alternative names
- CIG antibody
- Cold insoluble globulin antibody
- Cold-insoluble globulin antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab23751 has been referenced in 20 publications.
- Imafuku A et al. Rat Mesenchymal Stromal Cell Sheets Suppress Renal Fibrosis via Microvascular Protection. Stem Cells Transl Med 8:1330-1341 (2019). PubMed: 31675167
- Li Y et al. Early intervention with mesenchymal stem cells prevents nephropathy in diabetic rats by ameliorating the inflammatory microenvironment. Int J Mol Med 41:2629-2639 (2018). PubMed: 29484379
- Wilck N et al. Nitric oxide-sensitive guanylyl cyclase stimulation improves experimental heart failure with preserved ejection fraction. JCI Insight 3:N/A (2018). PubMed: 29467337
- Wang JL et al. Antifibrotic role of PGC-1a-siRNA against TGF-ß1-induced renal interstitial fibrosis. Exp Cell Res 370:160-167 (2018). PubMed: 29913155
- He WM et al. Oleanolic acid attenuates TGF-ß1-induced epithelial-mesenchymal transition in NRK-52E cells. BMC Complement Altern Med 18:205 (2018). PubMed: 29973206