Overview

  • Product name
  • Description
    Rabbit polyclonal to Fibronectin
  • Host species
    Rabbit
  • Specificity
    Fibronectin 100%, collagen type I, III and IV <0.1%, elastin <0.1%
  • Tested applications
    Suitable for: ELISA, IHC-P, RIA, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human, Pig
  • Immunogen

    Full length native protein (purified) corresponding to Rat Fibronectin.

  • Positive control
    • Rat skin
  • General notes


    This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.

Properties

Applications

Our Abpromise guarantee covers the use of ab23751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
RIA 1/5000.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • Tissue specificity
    Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • Involvement in disease
    Glomerulopathy with fibronectin deposits 2
  • Sequence similarities
    Contains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • Developmental stage
    Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • Post-translational
    modifications
    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylated by FAM20C in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • Cellular localization
    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CIG antibody
    • Cold insoluble globulin antibody
    • Cold-insoluble globulin antibody
    • DKFZp686F10164 antibody
    • DKFZp686H0342 antibody
    • DKFZp686I1370 antibody
    • DKFZp686O13149 antibody
    • ED B antibody
    • Fibronectin 1 antibody
    • FINC antibody
    • FINC_HUMAN antibody
    • FN antibody
    • FN1 antibody
    • FNZ antibody
    • GFND antibody
    • GFND2 antibody
    • LETS antibody
    • Migration stimulating factor antibody
    • MSF antibody
    • Ugl-Y3 antibody
    see all

Images

  • ab23751 at 1/200 dilution staining pig fibroblast cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with serum prior to incubation with antibody for 8 hours at 4°C. An Alexa-Fluor ® 488 conjugated  donkey anti-rabbit antibody was used as the secondary. The image was taken using a confocal laser scanning microscope and shows the presence of abundant fibronectin (green) in the pig fibroblasts. DAPI was used as nuclear counterstain (Blue).

    See Abreview

  • ab23751 staining rat intestine tissue section by immunohistochemistry (Formalin/PFA-fixed, paraffin-embbeded sections). Tissue underwent fixation in Bouins solution and processed by enzyme mediated antigen retrieval method. The primary antibody was diluted 1/500 and incubated with sample for 1 hour at 20°C. A HRP-conjugated mouse polyclonal to rabbit IgG was used undiluted as the secondary.

    See Abreview

  • ab23751 staining Fibronectin in Rat blood vessel tissue sections by IHC-P (formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 100% DEEB block for 10 minutes at room temperature. Enzymatic antigen retrieval was performed. Samples were incubated with primary antibody (1/1000) for 20 minutes at room temperature. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Li Y  et al. Early intervention with mesenchymal stem cells prevents nephropathy in diabetic rats by ameliorating the inflammatory microenvironment. Int J Mol Med 41:2629-2639 (2018). Read more (PubMed: 29484379) »
  • Wilck N  et al. Nitric oxide-sensitive guanylyl cyclase stimulation improves experimental heart failure with preserved ejection fraction. JCI Insight 3:N/A (2018). Read more (PubMed: 29467337) »
See all 17 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Pancreatic cancer cell line)
Permeabilization
Yes - saponin
Specification
Pancreatic cancer cell line
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 30 2018

Answer

Thank you for contacting us. This product was created using Purified fibronectin from rat plasma as immunogen. As a polyclonal antibody, multiple epitope sites will be recognized throughout the target. It may be that this antibody will target soluble and insoluble fibronectin however we do have evidence that the soluble form is recognized in porcine in an Abreview posted for this product. This Abreview may be found at the following location: https://www.abcam.com/Fibronectin-antibody-ab23751-reviews.html?intabreviewid=5890 Another porcine review may be found at: https://www.abcam.com/Fibronectin-antibody-ab23751-reviews.html?intabreviewid=12535 As we are not fibronectin experts, we cannot know whether the oncofetal fibronectin produced by the trophoblast has the same molecular structure. We recommend reviewing available literature to determine this. It does appear that best results are achieved when using enzymatic antigen retrieval. While use of heat mediated antigen retrieval using citrate buffer may work for product, we do not have knowledge of the effectiveness of this system.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Renal Proximal Tubular Epithelial cells)
Specification
Renal Proximal Tubular Epithelial cells
Fixative
Paraformaldehyde
Permeabilization
Yes - triton x100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 4°C

Ms. pei-fang Hsieh

Verified customer

Submitted Jul 19 2012

Answer

Thank you for your enquiry.

I can confirm that ab23751 fibronectin antibody is tested and therefore covered by our 6 month guarantee for IHC-P and rat and pig samples. Please note that ab131065 is tested and guaranteed in IHC-P and rat, but not in pig. Tested and guaranteed species and application information are provided on the online datasheets.

I can confirm the antibodies will detect fibronectin whether it is extracellular or intracellular.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

Answer

Thank you for your enquiry.

I am sorry to confirm that ab23751 has not yet been tested in Western blot. I am pleased to hear the customer would like to make use of our testing discount program.

I can provide the following code for the customer to test ab23751 in WB:

DISCOUNT CODE: ####
Expiration date: 27th July 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forWB and include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

Read More

Answer

Thank you for contacting us.  The fibronectin antibody ab23751 does indeed detect both the soluble and the insoluble form. The immunogen used to generate this antibody was fibronectin purified from plasma, i.e. the soluble fibronectin. The insoluble cell surface fibronectin can also be stained, as for example demonstrated by the abreviews. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (blood vessels)
Specification
blood vessels
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K DAKO S3020
Permeabilization
No
Blocking step
DEEB DAKO S2003 as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Trachea)
Specification
Trachea
Fixative
Bouins
Antigen retrieval step
Enzymatic
Permeabilization
No

Abcam user community

Verified customer

Submitted Nov 03 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Intestine)
Specification
Intestine
Fixative
Bouins
Antigen retrieval step
Enzymatic
Permeabilization
No

Abcam user community

Verified customer

Submitted Nov 03 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (Fibroblast)
Specification
Fibroblast
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%

Dr. Mal Niladri

Verified customer

Submitted Oct 16 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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