Anti-Fibronectin antibody (ab23751)

Thank you for contacting us. This product was created using Purified fibronectin from rat plasma as immunogen. As a polyclonal antibody, multiple epitope sites will be recognized throughout the target. It may be that this antibody will target soluble and insoluble fibronectin however we do have evidence that the soluble form is recognized in porcine in an Abreview posted for this product. This Abreview may be found at the following location: https://www.abcam.com/Fibronectin-antibody-ab23751-reviews.html?intabreviewid=5890 Another porcine review may be found at: https://www.abcam.com/Fibronectin-antibody-ab23751-reviews.html?intabreviewid=12535 As we are not fibronectin experts, we cannot know whether the oncofetal fibronectin produced by the trophoblast has the same molecular structure. We recommend reviewing available literature to determine this. It does appear that best results are achieved when using enzymatic antigen retrieval. While use of heat mediated antigen retrieval using citrate buffer may work for product, we do not have knowledge of the effectiveness of this system.

Thank you for your enquiry.

I can confirm that ab23751 fibronectin antibody is tested and therefore covered by our 6 month guarantee for IHC-P and rat and pig samples. Please note that ab131065 is tested and guaranteed in IHC-P and rat, but not in pig. Tested and guaranteed species and application information are provided on the online datasheets.

I can confirm the antibodies will detect fibronectin whether it is extracellular or intracellular.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Thank you for your enquiry.

I am sorry to confirm that ab23751 has not yet been tested in Western blot. I am pleased to hear the customer would like to make use of our testing discount program.

I can provide the following code for the customer to test ab23751 in WB:

DISCOUNT CODE: ####
Expiration date: 27th July 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forWB and include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

Thank you for contacting us.  The fibronectin antibody ab23751 does indeed detect both the soluble and the insoluble form. The immunogen used to generate this antibody was fibronectin purified from plasma, i.e. the soluble fibronectin. The insoluble cell surface fibronectin can also be stained, as for example demonstrated by the abreviews. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.