Anti-Fibronectin antibody (ab23752)

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Key features and details

  • Rabbit polyclonal to Fibronectin
  • Reacts with: Cow
  • Isotype: IgG

Overview

  • Product name

    Anti-Fibronectin antibody
    See all Fibronectin primary antibodies

  • Description

    Rabbit polyclonal to Fibronectin

  • Host species

    Rabbit

  • Species reactivity

    Reacts with: Cow

  • Immunogen

    Purified fibronectin from bovine plasma.

  • Positive control

    • Bovine skin

  • General notes


    This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Target

  • Function

    Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.

  • Tissue specificity

    Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.

  • Involvement in disease

    Glomerulopathy with fibronectin deposits 2

  • Sequence similarities

    Contains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.

  • Developmental stage

    Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.

  • Post-translational
    modifications

    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylated by FAM20C in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.

  • Cellular localization

    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt

  • Database links

    • Alternative names

      • CIG antibody
      • Cold insoluble globulin antibody
      • Cold-insoluble globulin antibody
      • DKFZp686F10164 antibody
      • DKFZp686H0342 antibody
      • DKFZp686I1370 antibody
      • DKFZp686O13149 antibody
      • ED B antibody
      • Fibronectin 1 antibody
      • FINC antibody
      • FINC_HUMAN antibody
      • FN antibody
      • FN1 antibody
      • FNZ antibody
      • GFND antibody
      • GFND2 antibody
      • LETS antibody
      • Migration stimulating factor antibody
      • MSF antibody
      • Ugl-Y3 antibody
      see all

    References (0)

    Publishing research using ab23752? Please let us know so that we can cite the reference in this datasheet.

    ab23752 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    Should I re-hydrate the sections after de-waxing and before the hyaluronidase treatment? Or should I treat with the hyaluronidase immediately after de-waxing?

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    Answer

    Thank you for your call today and for your patience. I have checked with my colleagues, and you should fully de-wax and re-hydrate the tissue prior to treating with the hyaluronidase. I hope this information is helpful, but please let me know if you have any further questions or if anything is unclear.

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    Question

    Hi, I am doing IHC-P with ab23752. I would like to know if there were any specific requirements for the hyaluronidase, such as species?

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    Answer

    Thanks for your inquiry. Bovine hyaluronidase will work as long as the enzyme activity is within the range given on the data sheet - "0.2% hyaluronidase (app. 300 U/mg)". I hope this helps. Please feel free to contact us if you require further assistance.

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