Overview

  • Product name

  • Description

    Rabbit polyclonal to Fibronectin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IP, IHC-FoFr, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Hamster, Cow, Dog, Human, African green monkey, Chinese hamster, Syrian hamster
  • Immunogen

    Fibronectin isolated from a pool of normal human plasma.

  • Positive control

    • IHC-Fr: Mouse colon and embryonic heart tissue. IHC-P: Human kidney tissue. WB: Human colon lysate; HepG2 and NIH/3T3 cell lysate. ICC/IF: HeLa cells.
  • General notes

      

Properties

Applications

Our Abpromise guarantee covers the use of ab2413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 285 kDa (predicted molecular weight: 262 kDa).
IP Use at an assay dependent concentration.

Used at 1ug/ml for 1 hr 30 min on renal carcinoma cell line 786-0 (see Abreview).

IHC-FoFr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

This antibody may be diluted to a titer of 1/50 - 1/250 in an ABC method. We suggest an incubation period of 30 minutes at room temperature.

Target

  • Function

    Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • Tissue specificity

    Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • Involvement in disease

    Glomerulopathy with fibronectin deposits 2
  • Sequence similarities

    Contains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • Developmental stage

    Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • Post-translational
    modifications

    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylated by FAM20C in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • Cellular localization

    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • CIG antibody
    • Cold insoluble globulin antibody
    • Cold-insoluble globulin antibody
    • DKFZp686F10164 antibody
    • DKFZp686H0342 antibody
    • DKFZp686I1370 antibody
    • DKFZp686O13149 antibody
    • ED B antibody
    • Fibronectin 1 antibody
    • FINC antibody
    • FINC_HUMAN antibody
    • FN antibody
    • FN1 antibody
    • FNZ antibody
    • GFND antibody
    • GFND2 antibody
    • LETS antibody
    • Migration stimulating factor antibody
    • MSF antibody
    • Ugl-Y3 antibody
    see all

Images

  • Paraformaldehyde-fixed frozen section of mouse colon tissue stained for Fibronectin (green) using ab2413 at 1/200 dilution in immunohistochemical analysis, followed by Alexa Fluor® 488 conjugated Goat anti-Rabbit IgG (H+L).

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, staining Fibronectin with ab2413.
  • All lanes : Anti-Fibronectin antibody (ab2413) at 1 µg/ml

    Lane 1 : Human colon tissue lysate - total protein (ab30051)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 262 kDa
    Observed band size: 285 kDa
    why is the actual band size different from the predicted?

  • Paraformaldehyde-fixed frozen section of mouse embryonic heart tissue stained for Fibronectin (green) using ab2413 at 1/100 dilution in immunohistochemical analysis, followed by Alexa Fluor® 488 conjugated Donkey anti-Rabbit IgG.

    Nuclear counterstain: DAPI (blue).

    See Abreview

  • ICC/IF image of ab2413 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2413, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.
  • ab2413 staining Fibronectin in human kidney tissue section by IHC-P (Formalin/PFA-fixed paraffin embedded tissue sections). Tissue sections were incubated with ab2413 at a dilution of 1:250 for one hour. Heat mediated antigen retrieval technique was used with citrate buffer at pH 6.0. DAB staining was done with a biotinylated secondary for 45 min at RT at a concentration of 1:1000.

References

This product has been referenced in:

  • Murphy S  et al. Proteomic analysis of the sarcolemma-enriched fraction from dystrophic mdx-4cv skeletal muscle. J Proteomics 191:212-227 (2019). Read more (PubMed: 29408692) »
  • Dye DE  et al. Interaction Between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System. Methods Mol Biol 1889:185-212 (2019). Read more (PubMed: 30367415) »
See all 267 Publications for this product

Customer reviews and Q&As

41-50 of 83 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (3T3 cells)
Specification
3T3 cells
Fixative
Formaldehyde
Permeabilization
Yes - Triton (0.1%) in PBS, 3min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 02 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Fixative
Formaldehyde
Permeabilization
Yes - Triton (0.1%) in PBS, 3min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 02 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (NRK cells)
Specification
NRK cells
Fixative
Formaldehyde
Permeabilization
Yes - Triton (0.1%) in PBS, 3min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 02 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (MDCK)
Specification
MDCK
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS for 3min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 01 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Syrian Hamster Cell (BHK)
Specification
BHK
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS for 3min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 01 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Chinese Hamster Cell (CHO)
Specification
CHO
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS for 3min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 01 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
African Green Monkey Cell (COS)
Specification
COS
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS for 3min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 01 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (NRK)
Specification
NRK
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS for 3min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 01 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (3T3)
Specification
3T3
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS for 3min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 01 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS for 3min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 01 2012

41-50 of 83 Abreviews or Q&A

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