Overview

  • Product name
  • Description
    Rabbit polyclonal to Fibronectin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-FoFr, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Cow, Dog, Human, African green monkey, Chinese hamster, Syrian hamster
  • Immunogen

    Fibronectin isolated from a pool of normal human plasma.

  • Positive control
    • IHC-Fr: Mouse colon and embryonic heart tissue. IHC-P: Human kidney tissue. WB: Human colon lysate; HepG2 and NIH/3T3 cell lysate. ICC/IF: HeLa cells.
  • General notes

    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

    See other anti-rabbit secondary antibodies that can be used with this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab2413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 285 kDa (predicted molecular weight: 262 kDa).
IP Use at an assay dependent concentration.

Used at 1ug/ml for 1 hr 30 min on renal carcinoma cell line 786-0 (see Abreview).

IHC-FoFr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

This antibody may be diluted to a titer of 1/50 - 1/250 in an ABC method. We suggest an incubation period of 30 minutes at room temperature.

Target

  • Function
    Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • Tissue specificity
    Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • Involvement in disease
    Glomerulopathy with fibronectin deposits 2
  • Sequence similarities
    Contains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • Developmental stage
    Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • Post-translational
    modifications
    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylated by FAM20C in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • Cellular localization
    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CIG antibody
    • Cold insoluble globulin antibody
    • Cold-insoluble globulin antibody
    • DKFZp686F10164 antibody
    • DKFZp686H0342 antibody
    • DKFZp686I1370 antibody
    • DKFZp686O13149 antibody
    • ED B antibody
    • Fibronectin 1 antibody
    • FINC antibody
    • FINC_HUMAN antibody
    • FN antibody
    • FN1 antibody
    • FNZ antibody
    • GFND antibody
    • GFND2 antibody
    • LETS antibody
    • Migration stimulating factor antibody
    • MSF antibody
    • Ugl-Y3 antibody
    see all

Images

  • Paraformaldehyde-fixed frozen section of mouse colon tissue stained for Fibronectin (green) using ab2413 at 1/200 dilution in immunohistochemical analysis, followed by Alexa Fluor® 488 conjugated Goat anti-Rabbit IgG (H+L).

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, staining Fibronectin with ab2413.
  • All lanes : Anti-Fibronectin antibody (ab2413) at 1 µg/ml

    Lane 1 : Human colon tissue lysate - total protein (ab30051)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 262 kDa
    Observed band size: 285 kDa
    why is the actual band size different from the predicted?

  • Paraformaldehyde-fixed frozen section of mouse embryonic heart tissue stained for Fibronectin (green) using ab2413 at 1/100 dilution in immunohistochemical analysis, followed by Alexa Fluor® 488 conjugated Donkey anti-Rabbit IgG.

    Nuclear counterstain: DAPI (blue).

    See Abreview

  • ICC/IF image of ab2413 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2413, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.
  • ab2413 staining Fibronectin in human kidney tissue section by IHC-P (Formalin/PFA-fixed paraffin embedded tissue sections). Tissue sections were incubated with ab2413 at a dilution of 1:250 for one hour. Heat mediated antigen retrieval technique was used with citrate buffer at pH 6.0. DAB staining was done with a biotinylated secondary for 45 min at RT at a concentration of 1:1000.

References

This product has been referenced in:
  • Imamura M  et al. RIPK3 promotes kidney fibrosis via AKT-dependent ATP citrate lyase. JCI Insight 3:N/A (2018). Read more (PubMed: 29415885) »
  • Steigedal TS  et al. Nephronectin is Correlated with Poor Prognosis in Breast Cancer and Promotes Metastasis via its Integrin-Binding Motifs. Neoplasia 20:387-400 (2018). WB . Read more (PubMed: 29539586) »
See all 192 Publications for this product

Customer reviews and Q&As

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1-10 of 63 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Vascular smooth muscle cell)
Permeabilization
No
Specification
Vascular smooth muscle cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 12 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (Vascular smooth muscle cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Vascular smooth muscle cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Dec 12 2018

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse colon tissue)
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
20 µg
Specification
mouse colon tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 11 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Spinal Cord)
Antigen retrieval step
None
Permeabilization
Yes - Triton X100
Specification
Spinal Cord
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4 · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 31 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (fibroblast)
Permeabilization
Yes - 0.1% triton x-100
Specification
fibroblast
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 10 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Sheep Tissue sections (Artery)
Permeabilization
Yes - 0.1% Triton X-100, 10 min
Specification
Artery
Blocking step
Serum as blocking agent for 4 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Ms. Chelsea Stowell

Verified customer

Submitted Mar 07 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (lung fibroblast)
Permeabilization
No
Specification
lung fibroblast
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 28 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
Liver
Blocking step
BSA + Milk + BFS as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 27°C
Fixative
Formaldehyde

Dr. Marcelo Pelajo Machado

Verified customer

Submitted May 25 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
spleen
Fixative
Formaldehyde

Dr. Marcelo Pelajo Machado

Verified customer

Submitted May 25 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic Heart)
Permeabilization
Yes - Tween-20
Specification
Embryonic Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 11 2016

1-10 of 63 Abreviews

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