Western blot abreview for Anti-Fibronectin antibody

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Abreviews
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (COS cells)
Loading amount
100 µg
Specification
COS cells
Gel Running Conditions
Reduced Denaturing (Tris-Glycine -10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA (5%) in TBS-T (0.5%)

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L)-HRP Conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
ECL+
Exposure
20 second(s)
Bands
Specific: 285 kDa
Positive control
COS cell lysate

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Submitted Oct 31 2012

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