Immunohistochemistry (Frozen sections) abreview for Anti-Fibronectin antibody

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic Heart)
Permeabilization
Yes - Tween-20
Specification
Embryonic Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST (1%BSA+0.2%Tween-20)
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti Rabbit Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
This antibody specifically stains fibronectin, which is an extra cellular matrix protein. As a result, the cell boundary of cardiomyocytes was clearly stained.

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Submitted Oct 11 2016

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