• Product name
  • Description
    Rabbit polyclonal to Fibronectin
  • Host species
  • Specificity
    This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Minimum Cross Reactivity to collagens and non-collagen extracellular matrix proteins.
  • Tested applications
    Suitable for: ELISA, IP, WB, ICC/IF, IHC-P, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration.

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human breast adenocarcinoma



Our Abpromise guarantee covers the use of ab299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IP 1/4000 - 1/8000.
WB 1/4000 - 1/8000.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [HFN 7.1] to Fibronectin - BSA and Azide free (ab80923) as Capture.


  • Function
    Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • Tissue specificity
    Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • Involvement in disease
    Glomerulopathy with fibronectin deposits 2
  • Sequence similarities
    Contains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • Developmental stage
    Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • Post-translational
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylated by FAM20C in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • Cellular localization
    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CIG antibody
    • Cold insoluble globulin antibody
    • Cold-insoluble globulin antibody
    • DKFZp686F10164 antibody
    • DKFZp686H0342 antibody
    • DKFZp686I1370 antibody
    • DKFZp686O13149 antibody
    • ED B antibody
    • Fibronectin 1 antibody
    • FINC antibody
    • FINC_HUMAN antibody
    • FN antibody
    • FN1 antibody
    • FNZ antibody
    • GFND antibody
    • GFND2 antibody
    • LETS antibody
    • Migration stimulating factor antibody
    • MSF antibody
    • Ugl-Y3 antibody
    see all


  • IHC image of Fibronectin antibody staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab299, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Standard Curve for Fibronectin (Analyte: Fibronectin protein (Human) (ab81743)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [HFN 7.1] to Fibronectin - BSA and Azide free (ab80923) at 1µg/ml and Detector Antibody Rabbit polyclonal to Fibronectin (ab299) at 0.5µg/ml.
  • ICC/IF image of ab299 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab299, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Li X  et al. Upregulation of lactate-inducible snail protein suppresses oncogene-mediated senescence through p16INK4ainactivation. J Exp Clin Cancer Res 37:39 (2018). Read more (PubMed: 29482580) »
  • Tanos T  et al. Isolation of putative stem cells present in human adult olfactory mucosa. PLoS One 12:e0181151 (2017). Read more (PubMed: 28719644) »
See all 17 Publications for this product

Customer reviews and Q&As

1-10 of 12 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sheep Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 15 2017


I found a good set of products that gives a strong sandwich ELISA signal.

Capture: ab80923

Detection: ab299

Antigen: ab81743

Click here (or use the following: https://www.abcam.com/index.html?datasheet=80923).

Click here (or use the following: https://www.abcam.com/index.html?datasheet=299).

Click here (or use the following: https://www.abcam.com/index.html?datasheet=81743).

The capture antibody can be easily biotinylated with one of our EasyLink kits. They come in three sizes:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=102864).

Click here (or use the following: https://www.abcam.com/index.html?datasheet=102865).

Click here (or use the following: https://www.abcam.com/index.html?datasheet=102863).

If you need an anti-rabbit IgG secondary antibody conjugated to gold, whe have several of these in three particle sizes: 6, 12, and 18 nm. For an example, please see the datasheet for ab105296:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=105296).

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I've included a list of capture, detection, protein, and fluorescent-conjugated secondary below against Fibronectin. The capture and detection antibodies have been tested and guaranteed to work in sandwich ELISA to detect human fibronectin:

Capture: ab80923 https://www.abcam.com/fibronectin-antibody-hfn-7-1-bsa-and-azide-free-ab80923.html
Detection: ab299 https://www.abcam.com/fibronectin-antibody-ab299.html
Fibronectin protein: ab81743 https://www.abcam.com/fibronectin-protein-human-ab81743.html
Fluorescent-conjugated secondary: any anti-rabbit fluorescent-conjugated IgG secondary, list https://www.abcam.com/Search?AppliedFacets.FacetProductType=Secondary+antibodies&AppliedFacets.FacetTargetName=IgG&AppliedFacets.FacetConjugate=DyLight%C2%AE+488&AppliedFacets.FacetConjugate=DyLight%C2%AE+550&AppliedFacets.FacetConjugate=DyLight%C2%AE+594&AppliedFacets.FacetConjugate=Chromeo%E2%84%A2+546&AppliedFacets.FacetConjugate=Cy2+%C2%AE&AppliedFacets.FacetConjugate=Cy3.5+%C2%AE&AppliedFacets.FacetConjugate=Cy5+%C2%AE&AppliedFacets.FacetConjugate=Cy5.5+%C2%AE&AppliedFacets.FacetConjugate=DyLight%C2%AE+650&AppliedFacets.FacetConjugate=FITC&AppliedFacets.FacetConjugate=Phycoerythrin&AppliedFacets.FacetConjugate=SureLight%C2%AE+Allophycocyanin&AppliedFacets.FacetConjugate=Texas+Red+%C2%AE&AppliedFacets.FacetConjugate=TRITC&AppliedFacets.FacetReactivity=Rabbit&IsFacetsOnly=False&PageNumber=1&Keywords%5b0%5d=anti-rabbit&X-Requested-With=XMLHttpRequest

The specific sandwich ELISA protocol used to validate these 2 primary antibodies using ab81743 Fibronectin protein as the target is attached to this email.

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Thank you for your phone call.

Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab299 in IHC-P. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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Thank you for your enquiry. We have several antibodies suitable for the labelling of macrophages/monocytes. However, unfortunately many of them have not been tested for theur use in mice. We do have an antibody that recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages; ab6640 F4/80 antibody [CI:A3-1]. This antibody is a rat monoclonal suitable for application in the following applications; FACS, IHC-Fr, IHC-P, IHC-R, IP, RIA, WB. It has received some very favourable reviews from our customers. Given that it is rat as opposed to mouse; this should prevent excessive background from IHC. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More


Thank you for your email. All the information that we have regarding these two antibodies is located on our online datasheets; we do not have any images available at this time. For ab292 I would recommend decreasing the concentration of the primary antibody - for Western blotting we recommend using ab292 at 1:5,000-1:10,000. Also, please note that greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used. For ab299, the antibody (immunoblot) shows a 450kDa band non-reduced and 225 kDa band when fully reduced, and a working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested. Also, please take a look at the reviews located on the datasheet. If you have any more questions, please contact us again.

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This antibody has been used in ELISA by using 100uL unlabeled antibody per well at a concentration of 10ug/mL.

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This antibody has not been tested in the application or species you describe above, therefore we can't guarantee results. Thesit, triton X100 or Tween 20 are classical solubilizing agents that should work well.

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This antibody, used in the applications listed and with the appropriate fluorescent-label-conjugated-secondary antibody, should perform well in immunofluorescence.

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The antigen was prepared from Human Plasma. The antibody (immunoblot) shows a 450kDa band non-reduced and 225 kDa band when fully reduced.

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