Recombinant Anti-Fibronectin antibody [EPR19241-46] (ab199056)
![Western blot - Anti-Fibronectin antibody [EPR19241-46] (ab199056)](https://www.abcam.com/ps/products/199/ab199056/Images/ab199056-285833-anti-fibronectin-antibody-epr19241-46-western-blot.jpg "Western blot - Anti-Fibronectin antibody [EPR19241-46] (ab199056)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19241-46] to Fibronectin
- Suitable for: WB, ICC/IF, IP, IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
-
Product name
Anti-Fibronectin antibody [EPR19241-46]
See all Fibronectin primary antibodies -
Description
Rabbit monoclonal [EPR19241-46] to Fibronectin -
Host species
Rabbit -
Specificity
Western blot and IHC tests of human samples were negative.
-
Tested applications
Suitable for: WB, ICC/IF, IP, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Mouse and rat liver lysates; Mouse and rat plasma; Hepa1-6 whole cell lysate. IHC-P: Mouse and rat stomach tissues. ICC/IF: Hepa1-6 cells. Flow Cyt (intra): Hepa1-6 cells. IP: Mouse plasma.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19241-46 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 273 kDa (predicted molecular weight: 273 kDa).
|
|
| ICC/IF |
1/250.
|
|
| IP |
1/30.
|
|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| Flow Cyt (Intra) |
1/500.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 273 kDa (predicted molecular weight: 273 kDa). |
|
ICC/IF
1/250. |
|
IP
1/30. |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
Flow Cyt (Intra)
1/500. |
Target
-
Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. -
Tissue specificity
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. -
Involvement in disease
Glomerulopathy with fibronectin deposits 2 -
Sequence similarities
Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. -
Developmental stage
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. -
Post-translational
modificationsSulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
-
Database links
- Entrez Gene: 14268 Mouse
- Entrez Gene: 25661 Rat
- SwissProt: P11276 Mouse
- SwissProt: P04937 Rat
-
Alternative names
- CIG antibody
- Cold insoluble globulin antibody
- Cold-insoluble globulin antibody
see all
Images
-
Western blot - Anti-Fibronectin antibody [EPR19241-46] (ab199056)All lanes : Anti-Fibronectin antibody [EPR19241-46] (ab199056) at 1/1000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Rat liver lysate
Lane 3 : Mouse plasma
Lane 4 : Rat plasma
Lane 5 : Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate
Lysates/proteins at 10 µl/ml buffer per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 273 kDa
Observed band size: 220-273 kDa why is the actual band size different from the predicted?Exposure time : Lanes 1 and 2:4 seconds; Lanes 3,4 and 5:2 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 6994838, PMID 7516997, PMID: 21923916).
-
![Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [EPR19241-46] (ab199056)](https://www.abcam.com/ps/products/199/ab199056/Images/ab199056-285827-anti-fibronectin-antibody-epr19241-46-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [EPR19241-46] (ab199056)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hepa 1-6 (mouse hepatoma epithelial cell line) cells labeling Fibronectin with ab199056 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining on Hepa1-6 cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] (ab199056)](https://www.abcam.com/ps/products/199/ab199056/Images/ab199056-285829-anti-fibronectin-antibody-epr19241-46-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] (ab199056)Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Fibronectin with ab199056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the extracellular matrix of mouse stomach (PMID: 650151).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] (ab199056)](https://www.abcam.com/ps/products/199/ab199056/Images/ab199056-285828-anti-fibronectin-antibody-epr19241-46-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] (ab199056)Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Fironectin with ab199056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the extracellular matrix of rat stomach (PMID: 650151).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Flow Cytometry (Intracellular) - Anti-Fibronectin antibody [EPR19241-46] (ab199056)](https://www.abcam.com/ps/products/199/ab199056/Images/ab199056-285824-anti-fibronectin-antibody-epr19241-46-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Fibronectin antibody [EPR19241-46] (ab199056)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling Fibronectin with ab199056 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluorr® 488) at 1/2000 dilution was used as the secondary antibody.
-
![Immunoprecipitation - Anti-Fibronectin antibody [EPR19241-46] (ab199056)](https://www.abcam.com/ps/products/199/ab199056/Images/ab199056-285821-anti-fibronectin-antibody-epr19241-46-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Fibronectin antibody [EPR19241-46] (ab199056)Fibronectin was immunoprecipitated from 0.35 mg of mouse plasma with ab199056 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199056 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse plasma 10 µg (Input).
Lane 2: ab199056 IP in mouse plasma.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199056 in mouse plasma.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
-
![Anti-Fibronectin antibody [EPR19241-46] (ab199056)](https://www.abcam.com/ps/products/199/ab199056/Images/ab199056-7-benefits-of-recombinant-antibodies.png)
Anti-Fibronectin antibody [EPR19241-46] (ab199056)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (6)
ab199056 has been referenced in 6 publications.
- Armacki M et al. Protein Kinase D1, Reduced in Human Pancreatic Tumors, Increases Secretion of Small Extracellular Vesicles From Cancer Cells That Promote Metastasis to Lung in Mice. Gastroenterology 159:1019-1035.e22 (2020). PubMed: 32446697
- Wang XF et al. DLX5 gene regulates the Notch signaling pathway to promote glomerulosclerosis and interstitial fibrosis in uremic rats. J Cell Physiol 234:21825-21837 (2019). PubMed: 31297803
- Kang SH et al. Effects of tranilast on the epithelial-to-mesenchymal transition in peritoneal mesothelial cells. Kidney Res Clin Pract 38:472-480 (2019). PubMed: 31554027
- Yang G et al. Fucoxanthin Alleviates Oxidative Stress through Akt/Sirt1/FoxO3a Signaling to Inhibit HG-Induced Renal Fibrosis in GMCs. Mar Drugs 17:N/A (2019). PubMed: 31842414
- Cheng J et al. Inhibition of lncRNA Dlx6os1 decreases cell proliferation and fibrosis and increases cell apoptosis in diabetic nephropathy. Int J Clin Exp Pathol 11:3302-3309 (2018). PubMed: 31949705
- Martínez-Fábregas J et al. Lysosomal protease deficiency or substrate overload induces an oxidative-stress mediated STAT3-dependent pathway of lysosomal homeostasis. Nat Commun 9:5343 (2018). PubMed: 30559339
