Recombinant Anti-Fibronectin antibody [F1] (ab32419)
![Western blot - Anti-Fibronectin antibody [F1] (ab32419)](https://www.abcam.com/ps/products/32/ab32419/Images/ab32419-243971-anti-fibronectin-antibody-f1-western-blot.jpg "Western blot - Anti-Fibronectin antibody [F1] (ab32419)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [F1] to Fibronectin
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Fibronectin antibody [F1]
See all Fibronectin primary antibodies -
Description
Rabbit monoclonal [F1] to Fibronectin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human Fibronectin aa 1-2400. The exact sequence is proprietary.
Database link: P02751 -
Positive control
- WB: Human serum and plasma. SK-OV-3 and HepG2 cell lysate. IHC-P: Human breast carcinoma tissue. ICC/IF: HepG2 and human mesenchymal stem cells. Flow Cyt (intra): HepG2 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
F1 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- Alexa Fluor® 488 Anti-Fibronectin antibody [F1] (ab198933)
- Alexa Fluor® 647 Anti-Fibronectin antibody [F1] (ab198934)
- Anti-Fibronectin antibody [F1] - Low endotoxin, Azide free (ab219366)
- Anti-Fibronectin antibody [F1] - BSA and Azide free (ab271831)
- Alexa Fluor® 555 Anti-Fibronectin antibody [F1] (ab275110)
- Alexa Fluor® 594 Anti-Fibronectin antibody [F1] (ab275336)
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/20.
|
|
| WB | (3) |
1/1000. Predicted molecular weight: 263 kDa.
|
| IHC-P | (2) |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| ICC/IF | (1) |
1/150.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/20. |
|
WB
1/1000. Predicted molecular weight: 263 kDa. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/150. |
Target
-
Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. -
Tissue specificity
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. -
Involvement in disease
Glomerulopathy with fibronectin deposits 2 -
Sequence similarities
Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. -
Developmental stage
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. -
Post-translational
modificationsSulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
-
Database links
- Entrez Gene: 2335 Human
- Omim: 135600 Human
- SwissProt: P02751 Human
- Unigene: 203717 Human
-
Alternative names
- CIG antibody
- Cold insoluble globulin antibody
- Cold-insoluble globulin antibody
see all
Images
-
Western blot - Anti-Fibronectin antibody [F1] (ab32419)All lanes : Anti-Fibronectin antibody [F1] (ab32419) at 1/5000 dilution (purified)
Lane 1 : Human serum
Lane 2 : Human plasma
Lane 3 : SK-OV-3 (Human ovarian cancer cell line) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 263 kDa
Observed band size: 263 kDaBlocking/Dilution buffer: 5% NFDM/TBST
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [F1] (ab32419)](https://www.abcam.com/ps/products/32/ab32419/Images/ab32419-244120-anti-fibronectin-antibody-f1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [F1] (ab32419)Immunohistochemical staining of paraffin embedded human breast carcinoma with purified ab32419 at a dilution of 1/250. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
-
![Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F1] (ab32419)](https://www.abcam.com/ps/products/32/ab32419/Images/ab32419-44743-anti-fibronectin-antibody-f1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F1] (ab32419)ICC/IF image of unpurified ab32419 stained HepG2 (Human liver hepatocellular carcinoma cell line) cells. The cells were fixed in 4% formaldehyde (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab32419 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
Alexa Fluor® 488 (ab198933) and Alexa Fluor® 647 (ab198934) conjugated versions are available for this clone.
-
![Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F1] (ab32419)](https://www.abcam.com/ps/products/32/ab32419/Images/ab32419-44744-anti-fibronectin-antibody-f1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F1] (ab32419)This image is courtesy of an anonymous abreview.ICC/IF image of unpurified ab32419 stained human mesenchymal stem cells. The cells were fixed in paraformaldehyde and then incubated in 0.1%BSA / 1% goat serum for 30 minutes, to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32419, 1/100 dilution) for 2 hours at 22°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG. DAPI was used to stain the cell nuclei (blue).
-
![Flow Cytometry (Intracellular) - Anti-Fibronectin antibody [F1] (ab32419)](https://www.abcam.com/ps/products/32/ab32419/Images/ab32419-269422-anti-fibronectin-antibody-f1-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Fibronectin antibody [F1] (ab32419)Intracellular Flow Cytometry analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Fibronectin with purified ab32419 at 1/20 dilution (10µg/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
-
![Western blot - Anti-Fibronectin antibody [F1] (ab32419)](https://www.abcam.com/ps/products/32/ab32419/Images/ab32419-243972-anti-fibronectin-antibody-f1-western-blot.jpg)
Western blot - Anti-Fibronectin antibody [F1] (ab32419)Anti-Fibronectin antibody [F1] (ab32419) at 1/1000 dilution (purified) + HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 263 kDa
Observed band size: 263 kDaBlocking/Dilution buffer: 5% NFDM/TBST
-
![Anti-Fibronectin antibody [F1] (ab32419)](https://www.abcam.com/ps/products/32/ab32419/Images/ab32419-11-benefits-of-recombinant-antibodies.png)
Anti-Fibronectin antibody [F1] (ab32419)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (90)
ab32419 has been referenced in 90 publications.
- Xu D et al. TAK1 inhibition improves myoblast differentiation and alleviates fibrosis in a mouse model of Duchenne muscular dystrophy. J Cachexia Sarcopenia Muscle 12:192-208 (2021). PubMed: 33236534
- Yu R et al. Mevalonate Pathway Inhibition Slows Breast Cancer Metastasis via Reduced N-glycosylation Abundance and Branching. Cancer Res 81:2625-2635 (2021). PubMed: 33602786
- Wan F et al. TET2 mediated demethylation is involved in the protective effect of triptolide on podocytes. Am J Transl Res 13:1233-1244 (2021). PubMed: 33841652
- Mou K et al. Zwint facilitates melanoma progression by promoting c-Myc expression. Exp Ther Med 22:818 (2021). PubMed: 34131441
- Wang J et al. Anti-Fibrotic Effects of Low Toxic Microcystin-RR on Bleomycin-Induced Pulmonary Fibrosis: A Comparison with Microcystin-LR. Front Pharmacol 12:675907 (2021). PubMed: 34168562