Anti-Fibronectin antibody [Fn-3] (ab18265)

Submit a review Q&A (2)References (7)
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [Fn-3] (ab18265)

    Key features and details

    • Mouse monoclonal [Fn-3] to Fibronectin
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG1

    Overview

    • Product name

      Anti-Fibronectin antibody [Fn-3]
      See all Fibronectin primary antibodies

    • Description

      Mouse monoclonal [Fn-3] to Fibronectin

    • Host species

      Mouse

    • Specificity

      Reacts with human cellular fibronectin, but not plasma fibronectin.

    • Tested applications

      Suitable for: ICC/IFmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Tissue, cells or virus corresponding to Fibronectin. FR5 cells, derived by SV40 transformation of human mammary epithelial cells.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab18265 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    Use a concentration of 1 µg/ml.
    Notes
    ICC/IF
    Use a concentration of 1 µg/ml.

    Target

    • Function

      Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
      Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.

    • Tissue specificity

      Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.

    • Involvement in disease

      Glomerulopathy with fibronectin deposits 2

    • Sequence similarities

      Contains 12 fibronectin type-I domains.
      Contains 2 fibronectin type-II domains.
      Contains 16 fibronectin type-III domains.

    • Developmental stage

      Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.

    • Post-translational
      modifications

      Sulfated.
      It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
      Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
      Phosphorylated by FAM20C in the extracellular medium.
      Proteolytic processing produces the C-terminal NC1 peptide, anastellin.

    • Cellular localization

      Secreted, extracellular space, extracellular matrix.
    • Information by UniProt

    • Database links

      • Alternative names

        • CIG antibody
        • Cold insoluble globulin antibody
        • Cold-insoluble globulin antibody
        • DKFZp686F10164 antibody
        • DKFZp686H0342 antibody
        • DKFZp686I1370 antibody
        • DKFZp686O13149 antibody
        • ED B antibody
        • Fibronectin 1 antibody
        • FINC antibody
        • FINC_HUMAN antibody
        • FN antibody
        • FN1 antibody
        • FNZ antibody
        • GFND antibody
        • GFND2 antibody
        • LETS antibody
        • Migration stimulating factor antibody
        • MSF antibody
        • Ugl-Y3 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [Fn-3] (ab18265)
        Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [Fn-3] (ab18265)

        ICC/IF image of ab18265 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18265, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      References (7)

      Publishing research using ab18265? Please let us know so that we can cite the reference in this datasheet.

      ab18265 has been referenced in 7 publications.

      • Wei M  et al. Genipin Induces Autophagy and Suppresses Cell Growth of Oral Squamous Cell Carcinoma via PI3K/AKT/MTOR Pathway. Drug Des Devel Ther 14:395-405 (2020). PubMed: 32099325
      • Wang J  et al. Long noncoding RNA homeobox A11 antisense promotes transforming growth factor ß1-induced fibrogenesis in cardiac fibroblasts. Mol Med Rep 19:2817-2824 (2019). PubMed: 30720066
      • Gan L  et al. Long non-coding RNA H19 regulates cell growth and metastasis via the miR-22-3p/Snail1 axis in gastric cancer. Int J Oncol 54:2157-2168 (2019). PubMed: 31081061
      • Wong CH  et al. Bio-functionalized magnetic nanoparticles for the immunoassay of fetal fibronectin: a feasibility study for the prediction of preterm birth. Sci Rep 7:42461 (2017). PubMed: 28198381
      • Bambang IF  et al. Endoplasmic reticulum protein 29 regulates epithelial cell integrity during the mesenchymal-epithelial transition in breast cancer cells. Oncogene : (2012). WB . PubMed: 22543584
      • Cseh B  et al. Autocrine fibronectin directs matrix assembly and crosstalk between cell-matrix and cell-cell adhesion in vascular endothelial cells. J Cell Sci 123:3989-99 (2010). ICC/IF ; Cow . PubMed: 20980391
      • Keen J  et al. Monoclonal antibodies that distinguish between human cellular and plasma fibronectin. Mol Biol Med 2:15-27 (1984). PubMed: 6083431

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      What is a good positive control tissue?

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      Answer

      Thank you for your enquiry. Here is a reference that includes the information you need: Keen J et al. 1984. Mol. Biol. Med. 2:15-27 Pub Med ID:6083431 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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      Question

      The above mentioned antibody can distinguish between the cellular and plasma FN. Hower neither the datasheet nor the references don't specify whether it recognises the EDA or EDB region (specific of cellular FN). Is there any possibility for your team to check, whether this is also specific to EDA as the IST-9 clone? If it isn't, then the suspicion is quite big, that it is EDB, which is recognised. If it is so, then Abcam could sell it as antibody against the Extra Domain B, being the only commercialised antibody of this kind. I think besides me, there are more scientists to use an EDB antibody. If for Abcam, there are no opportunity to check this, I can offer myself to make this assesment. We already have your EDA antibody to compare with. We have a well working silencing system for FN variants. I hope we can both benefit from this antibody!

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      Answer

      Thank you for your enquiry. The Fn-3 clone recognizes a determinant on cellular but not plasma fibronectin. I am assuming this is the EDB region, although this is not explicitly stated in the reference I have. I will send this reference to you in a separate email. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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