Key features and details
- Mouse monoclonal [TV.1] to Fibronectin
- Suitable for: IP, Flow Cyt, WB, ICC/IF, IHC-Fr, IHC-P
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG1
Product nameAnti-Fibronectin antibody [TV.1]
See all Fibronectin primary antibodies
DescriptionMouse monoclonal [TV.1] to Fibronectin
Specificityab194395 reacts with the cellular as well as plasma form of Fibronectin.
Tested applicationsSuitable for: IP, Flow Cyt, WB, ICC/IF, IHC-Fr, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human, Pig
Tissue, cells or virus corresponding to Human Fibronectin. T-cell lymphoma biopsy
Database link: P02751
- SW620 cells or Kidney
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityProtein G purified
Purification notesPurified from Bioreactor Concentrate
Light chain typekappa
Our Abpromise guarantee covers the use of ab194395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 1-2 µg/mg of lysate.|
|Flow Cyt||Use 0.5-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 262 kDa.
Actual Band: 220kDa (monomer); 440kDa (dimer)
|ICC/IF||Use a concentration of 0.5 - 1 µg/ml.|
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
Involvement in diseaseGlomerulopathy with fibronectin deposits 2
Sequence similaritiesContains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains.
Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
Cellular localizationSecreted, extracellular space, extracellular matrix.
- Information by UniProt
- CIG antibody
- Cold insoluble globulin antibody
- Cold-insoluble globulin antibody
ab194395 has been referenced in 4 publications.
- Boel NM et al. LRP1 is required for novobiocin-mediated fibronectin turnover. Sci Rep 8:11438 (2018). PubMed: 30061663
- Belyanina IV et al. In Vivo Cancer Cells Elimination Guided by Aptamer-Functionalized Gold-Coated Magnetic Nanoparticles and Controlled with Low Frequency Alternating Magnetic Field. Theranostics 7:3326-3337 (2017). PubMed: 28900513
- Cao L et al. Effects of tanshinone IIA on the regulation of renal proximal tubular fibrosis. Mol Med Rep 15:4247-4252 (2017). PubMed: 28440499
- Guan Y et al. The effective bioengineering method of implantation decellularized renal extracellular matrix scaffolds. Oncotarget 6:36126-38 (2015). PubMed: 26418881