Key features and details
- Rabbit polyclonal to Fibulin 1
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-Fibulin 1 antibody
See all Fibulin 1 primary antibodies
DescriptionRabbit polyclonal to Fibulin 1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse
- IHC-P: Rat liver, testis, stomach and pancreas tissues. WB: Rat heart, liver and brain lysates; Recombinant Rat Fibulin 1 protein.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab230994 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab230994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 78 kDa.|
FunctionIncorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP.
Tissue specificityIsoform A and isoform B are only expressed in placenta. Isoform C and isoform D are expressed in a variety of tissues and cultured cells.
Involvement in diseaseNote=A chromosomal aberration involving FBLN1 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with C12orf2. Fibroblasts derived from a patient with synpolydactyly displayed alterations in the level of isoform D splice variant incorporated into the ECM and secreted into the conditioned culture medium. By contrast, the expression of isoform C was not perturbed in the patients fibroblasts. Furthermore, no aberrant polypeptides were detected in extracts of cultured patients fibroblasts. The translocation t(12;22) may result in haploinsufficiency of the isoform D splice variant, which could lead to the observed limb malformation.
Note=Elevated expression and altered processing of FBLN1 protein is associated with human breast cancer.
Sequence similaritiesBelongs to the fibulin family.
Contains 3 anaphylatoxin-like domains.
Contains 9 EGF-like domains.
Developmental stageWidely expressed during embryonic development. Prominent in the matrix of the leptomeningeal anlage, in basement membranes of the neuroepithelium and the perineurium of peripheral nerves. In embryos of gestational week (gw) 4, staining was observed in the early mesenchymal bone anlagen. In gw 6.5 and 8, all perichondrial structures showed expression but the chondrocytes themselves showed no staining. In gw 10, expression is prominent in the interterritorial matrix surrounding the hypertrophic chondrocytes.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Basement membrane protein 90 antibody
- BM 90 antibody
- FBLN antibody
Paraffin-embedded rat liver tissue stained for Fibulin 1 using ab230994 at 20 µg/ml in immunohistochemical analysis.
All lanes : Anti-Fibulin 1 antibody (ab230994) at 2 µg/ml
Lane 1 : Rat serum
Lane 2 : Rat heart lysate
Lane 3 : Rat liver lysate
Lane 4 : Rat brain lysate
Predicted band size: 78 kDa
Paraffin-embedded rat testis tissue stained for Fibulin 1 using ab230994 at 20 µg/ml in immunohistochemical analysis.
Paraffin-embedded rat stomach tissue stained for Fibulin 1 using ab230994 at 20 µg/ml in immunohistochemical analysis.
Paraffin-embedded rat pancreas tissue stained for Fibulin 1 using ab230994 at 20 µg/ml in immunohistochemical analysis.
Anti-Fibulin 1 antibody (ab230994) at 2 µg/ml + Recombinant Rat Fibulin 1 protein
Predicted band size: 78 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230994 has not yet been referenced specifically in any publications.