Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10913] to Fibulin 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-Fibulin 1 antibody [EPR10913]
See all Fibulin 1 primary antibodies
DescriptionRabbit monoclonal [EPR10913] to Fibulin 1
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human Fibulin 1 aa 250-450. The exact sequence is proprietary.
Database link: P23142
- Human placenta, fetal liver and fetal heart tissue lysate
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 77 kDa.|
FunctionIncorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP.
Tissue specificityIsoform A and isoform B are only expressed in placenta. Isoform C and isoform D are expressed in a variety of tissues and cultured cells.
Involvement in diseaseNote=A chromosomal aberration involving FBLN1 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with C12orf2. Fibroblasts derived from a patient with synpolydactyly displayed alterations in the level of isoform D splice variant incorporated into the ECM and secreted into the conditioned culture medium. By contrast, the expression of isoform C was not perturbed in the patients fibroblasts. Furthermore, no aberrant polypeptides were detected in extracts of cultured patients fibroblasts. The translocation t(12;22) may result in haploinsufficiency of the isoform D splice variant, which could lead to the observed limb malformation.
Note=Elevated expression and altered processing of FBLN1 protein is associated with human breast cancer.
Sequence similaritiesBelongs to the fibulin family.
Contains 3 anaphylatoxin-like domains.
Contains 9 EGF-like domains.
Developmental stageWidely expressed during embryonic development. Prominent in the matrix of the leptomeningeal anlage, in basement membranes of the neuroepithelium and the perineurium of peripheral nerves. In embryos of gestational week (gw) 4, staining was observed in the early mesenchymal bone anlagen. In gw 6.5 and 8, all perichondrial structures showed expression but the chondrocytes themselves showed no staining. In gw 10, expression is prominent in the interterritorial matrix surrounding the hypertrophic chondrocytes.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Basement membrane protein 90 antibody
- BM 90 antibody
- FBLN antibody
All lanes : Anti-Fibulin 1 antibody [EPR10913] (ab175204) at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : Fetal liver tissue lysate
Lane 3 : Fetal heart tissue lysate
Lysates/proteins at 1/10 dilution per lane.
Predicted band size: 77 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
ab175204 has been referenced in 2 publications.