Anti-Fibulin 2 antibody (ab234993)
Key features and details
- Rabbit polyclonal to Fibulin 2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Fibulin 2 antibody
See all Fibulin 2 primary antibodies -
Description
Rabbit polyclonal to Fibulin 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Fibulin 2 aa 300-450.
Database link: P98095 -
Positive control
- WB: HeLa, U-251 MG and A549 whole cell lysates. IHC-P: Human placenta and liver cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).
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IHC-P |
1/500 - 1/1000.
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Notes |
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WB
1/500 - 1/5000. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa). |
IHC-P
1/500 - 1/1000. |
Target
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Function
Its binding to fibronectin and some other ligands is calcium dependent. -
Tissue specificity
Component of both basement membranes and other connective tissues. Expressed in heart, placenta and ovary. -
Sequence similarities
Belongs to the fibulin family.
Contains 3 anaphylatoxin-like domains.
Contains 11 EGF-like domains. -
Developmental stage
Widely expressed during embryonic development. Primarily detected within the neuropithelium, spinal ganglia and peripheral nerves. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 2199 Human
- Omim: 135821 Human
- SwissProt: P98095 Human
- Unigene: 198862 Human
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Alternative names
- Fbln2 antibody
- FBLN2_HUMAN antibody
- FIBL-2 antibody
see all
Images
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Paraffin-embedded human placenta tissue stained for Fibulin 2 using ab234993 at 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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All lanes : Anti-Fibulin 2 antibody (ab234993) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 127 kDa
Observed band size: 127 kDa -
Paraffin-embedded human liver cancer tissue stained for Fibulin 2 using ab234993 at 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab234993 has not yet been referenced specifically in any publications.