Recombinant Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-Fibulin-4 antibody [EPR684(2)]
See all Fibulin-4 primary antibodies -
Description
Rabbit monoclonal [EPR684(2)] to Fibulin-4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Fibulin-4 aa 400-500. The exact sequence is proprietary.
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Positive control
- JAR and SW480 cell lysates, Human fetal lung lysate, Human breast carcinoma and muscle tissues.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as EFEMP2
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR684(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab125073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 49 kDa. | |
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Involvement in disease
Defects in EFEMP2 are a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100]. Hereditary cutis laxa refers to a heterogeneous group of connective tissue disorders characterized by cutaneous abnormalities and variable systemic manifestations. The most constant clinical feature is loose skin, sagging over the face and trunk. Hereditary cutis laxa is inherited in both autosomal dominant and autosomal recessive modes. ARCL1 shows the most severe phenotype and has the poorest prognosis. In addition to the skin, internal organs enriched in elastic fibers, such as the lung and arteries, are affected. -
Sequence similarities
Belongs to the fibulin family.
Contains 6 EGF-like domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 30008 Human
- Entrez Gene: 58859 Mouse
- Omim: 604633 Human
- SwissProt: O95967 Human
- SwissProt: Q9WVJ9 Mouse
- Unigene: 170622 Human
- Unigene: 276367 Mouse
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Alternative names
- EFEMP 2 antibody
- EFEMP2 antibody
- EGF containing fibulin like extracellular matrix protein 2 antibody
see all
Images
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![Western blot - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)](https://www.abcam.com/ps/products/125/ab125073/Images/ab125073-252270-anti-efemp2-antibody-epr6842-western-blot.jpg)
Western blot - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)All lanes : Anti-Fibulin-4 antibody [EPR684(2)] (ab125073) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) whole cell lysates
Lane 2 : HepG2 (human hepatocellular carcinoma) whole cell lysates
Lane 3 : Human fetal lung lysates
Lane 4 : Human colon cancer lysates
Lane 5 : Human placenta lysates
Lane 6 : Mouse placenta lysates
Lane 7 : Mouse lung lysates
Lane 8 : NIH/3T3 (mouse embryo) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 49 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)](https://www.abcam.com/ps/products/125/ab125073/Images/ab125073-139220-anti-efemp2-antibody-epr6842-western-blot.jpg)
Western blot - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)All lanes : Anti-Fibulin-4 antibody [EPR684(2)] (ab125073) at 1/1000 dilution
Lane 1 : JAR cell lysate
Lane 2 : SW480 cell lysate
Lane 3 : Human fetal lung lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 49 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)](https://www.abcam.com/ps/products/125/ab125073/Images/ab125073-139219-anti-efemp2-antibody-epr6842-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)ab125073, at 1/250, staining Fibulin-4 in paraffin-embedded Human breast carcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)](https://www.abcam.com/ps/products/125/ab125073/Images/ab125073-139218-anti-efemp2-antibody-epr6842-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] (ab125073)ab125073, at 1/250, staining Fibulin-4 in paraffin-embedded Human muscle tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Datasheets and documents
References
This product has been referenced in:
- Wang T et al. Fibulin-4 is associated with prognosis of endometrial cancer patients and inhibits cancer cell invasion and metastasis via Wnt/ß-catenin signaling pathway. Oncotarget 8:18991-19012 (2017). ICC ; Human . Read more (PubMed: 28177909) »
- Zhang D et al. Fibulin-4 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition via the PI3K/Akt/mTOR pathway. Int J Oncol 50:1513-1530 (2017). IHC-P, ICC/IF, WB ; Human . Read more (PubMed: 28339091) »
