Overview

  • Product name

  • Description

    Rabbit polyclonal to Fibulin 5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Fibulin 5 aa 126-206 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9UBX5

  • Positive control

    • Human placenta tissue; Human colon carcinoma lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab202977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.

(or 1/50 - 1/200 if using a fluorescent secondary antibody).

WB 1/100 - 1/1000. Predicted molecular weight: 50 kDa.

Target

  • Function

    Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Could be a vascular ligand for integrin receptors and may play a role in vascular development and remodeling.
  • Tissue specificity

    Expressed predominantly in heart, ovary, and colon but also in kidney, pancreas, testis, lung and placenta. Not detectable in brain, liver, thymus, prostate, or peripheral blood leukocytes.
  • Involvement in disease

    Defects in FBLN5 are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Hereditary cutis laxa refers to a heterogeneous group of connective tissue disorders characterized by cutaneous abnormalities and variable systemic manifestations. The most constant clinical feature is loose skin, sagging over the face and trunk. Hereditary cutis laxa is inherited in both autosomal dominant and autosomal recessive modes. Autosomal dominant cutis laxa is a relatively benign inherited and acquired connective tissue disorder.
    Defects in FBLN5 are a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100]. Hereditary cutis laxa refers to a heterogeneous group of connective tissue disorders characterized by cutaneous abnormalities and variable systemic manifestations. The most constant clinical feature is loose skin, sagging over the face and trunk. Hereditary cutis laxa is inherited in both autosomal dominant and autosomal recessive modes. ARCL1 shows the most severe phenotype and has the poorest prognosis. In addition to the skin, internal organs enriched in elastic fibers, such as the lung and arteries, are affected.
    Defects in FBLN5 are the cause of age-related macular degeneration type 3 (ARMD3) [MIM:608895]. ARMD is a multifactorial disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid (known as drusen) that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • Sequence similarities

    Belongs to the fibulin family.
    Contains 6 EGF-like domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADCL2 antibody
    • ARCL1A antibody
    • ARMD3 antibody
    • Dance antibody
    • Developmental arteries and neural crest EGF like protein antibody
    • Developmental arteries and neural crest EGF-like protein antibody
    • EVEC antibody
    • Fbln5 antibody
    • FBLN5_HUMAN antibody
    • FIBL 5 antibody
    • FIBL-5 antibody
    • Fibulin-5 antibody
    • FLJ90059 antibody
    • UP50 antibody
    • Urine p50 protein antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue sections labeling Fibulin 5 with ab202977 at 1/400 dilution. Antigen retrieval was performed by boiling in sodium citrate buffer (pH 6.0) for 15 minutes, followed by endogenous peroxidase blocking with 3% hydrogen peroxide for 20 minutes. The tissue was incubated with Anti-Fibulin 5 antibody (ab202977) at 1/400 dilution overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat ovary tissue sections labeling Fibulin 5 with ab202977 at 1/500 dilution. Antigen retrieval was performed by boiling in sodium citrate buffer (pH 6.0) for 15 minutes, followed by endogenous peroxidase blocking with 3% hydrogen peroxide for 20 minutes. The tissue was incubated with Anti-Fibulin 5 antibody (ab202977) at 1/500 dilution overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

     

  • All lanes : Anti-Fibulin 5 antibody (ab202977) at 1/200 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Human colon carcinoma lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling Fibulin 5 with ab202977 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

References

ab202977 has not yet been referenced specifically in any publications.

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