Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23146-225] to Ficolin 2 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
Product nameAnti-Ficolin 2 antibody [EPR23146-225] - BSA and Azide free
See all Ficolin 2 primary antibodies
DescriptionRabbit monoclonal [EPR23146-225] to Ficolin 2 - BSA and Azide free
Tested applicationsSuitable for: IP, WBmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Recombinant fragment within Human Ficolin 2 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: Q15485
- WB: Human plasma; human liver lysate; Human Ficolin-2 recombinant protein (aa26- 313). IP: Human plasma.
ab270404 is the carrier-free version of ab267473. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab270404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 35, 400 kDa (predicted molecular weight: 34 kDa).|
FunctionMay function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin. Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect via the collagen region.
Tissue specificityExpressed by the liver and secreted in plasma.
Sequence similaritiesBelongs to the ficolin lectin family.
Contains 1 collagen-like domain.
Contains 1 fibrinogen C-terminal domain.
DomainThe fibrinogen-like domain (FBG) contains potential calcium-binding sites that may be involved in carbohydrate binding.
- Information by UniProt
- 37 kDa elastin-binding protein antibody
- Collagen/fibrinogen domain containing protein 2 antibody
- Collagen/fibrinogen domain-containing protein 2 antibody
Ficolin 2 was immunoprecipitated from 0.35 mg Human plasma with ab267473 at 1/30 dilution . Western blot was performed on the immunoprecipitate using ab267473 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP(ab131366) was used at 1/5000 dilution.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 7.75 seconds
Ficolin 2 has a high molecular weight (250 kDa) due to extensive disulfide bridge formation (PMID: 16595153).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abXXX).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab270404 has not yet been referenced specifically in any publications.