Recombinant Anti-Ficolin 2 antibody [EPR23146-225] - BSA and Azide free (ab270404)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23146-225] to Ficolin 2 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Ficolin 2 antibody [EPR23146-225] - BSA and Azide free
See all Ficolin 2 primary antibodies -
Description
Rabbit monoclonal [EPR23146-225] to Ficolin 2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human plasma; human liver lysate; Human Ficolin-2 recombinant protein (aa26- 313). IP: Human plasma.
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General notes
ab270404 is the carrier-free version of ab267473.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23146-225 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 35, 400 kDa (predicted molecular weight: 34 kDa).
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Notes |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 35, 400 kDa (predicted molecular weight: 34 kDa). |
Target
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Function
May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin. Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect via the collagen region. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Sequence similarities
Belongs to the ficolin lectin family.
Contains 1 collagen-like domain.
Contains 1 fibrinogen C-terminal domain. -
Domain
The fibrinogen-like domain (FBG) contains potential calcium-binding sites that may be involved in carbohydrate binding. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2220 Human
- Omim: 601624 Human
- SwissProt: Q15485 Human
- Unigene: 54517 Human
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Alternative names
- 37 kDa elastin-binding protein antibody
- Collagen/fibrinogen domain containing protein 2 antibody
- Collagen/fibrinogen domain-containing protein 2 antibody
see all
Images
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Ficolin 2 was immunoprecipitated from 0.35 mg Human plasma with ab267473 at 1/30 dilution . Western blot was performed on the immunoprecipitate using ab267473 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP(ab131366) was used at 1/5000 dilution.
Lane 1: Human plasma lysate 10 μg.
Lane 2: ab267473 IP in Human plasma.
Lane 3: Rabbit monoclonal IgG isotype control (ab172730) instead of ab267473 in Human plasma.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 7.75 seconds
Ficolin 2 has a high molecular weight (250 kDa) due to extensive disulfide bridge formation (PMID: 16595153).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abXXX).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab270404 has not yet been referenced specifically in any publications.