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    fif-antibody-ab65836.pdf

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Cell Biology Apoptosis Intracellular Survivin / IAPs
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Anti-FIF antibody (ab65836)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)
  • Western blot - Anti-FIF antibody (ab65836)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)
  • Immunocytochemistry/ Immunofluorescence - Anti-FIF antibody (ab65836)
  • Immunohistochemistry (Frozen sections) - Anti-FIF antibody (ab65836)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)
  • Immunocytochemistry/ Immunofluorescence - Anti-FIF antibody (ab65836)

Key features and details

  • Rabbit polyclonal to FIF
  • Suitable for: ICC/IF, IHC-P, WB, IHC-Fr
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-FIF antibody
    See all FIF primary antibodies
  • Description

    Rabbit polyclonal to FIF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human FIF.
    Database link: Q9BZZ5

  • Positive control

    • WB: Rat Cardiac Muscle, Rat Brain, Rat Testis, Rat Placenta, MCF-7, HeLa, CEM, SMMC, and COLO320 Lysates. IHC-P: Rat Brain, Human placenta, Rat intestines, and Mouse intestines testis. IHC-F: Rat Cardiac Muscle. ICC/IF: HeLa cells.
  • General notes

    Previously labelled as Apoptosis inhibitor 5. 

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
    Constituents: 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate, 2.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Survivin / IAPs
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • IAPs
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • IAPs

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab65836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 0.5 - 1 µg/ml.
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB (1)
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IHC-Fr
Use a concentration of 0.5 - 1 µg/ml.
Notes
ICC/IF
Use a concentration of 0.5 - 1 µg/ml.
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IHC-Fr
Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function

    Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
  • Tissue specificity

    Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer.
  • Sequence similarities

    Belongs to the API5 family.
  • Domain

    Two regions, an N-terminal (aa 96-107) and a C-terminal (aa 274-311) are required for binding FGF2.
  • Cellular localization

    Cytoplasm and Nucleus. Cytoplasm. Mainly nuclear. Can also be cytoplasmic.
  • Target information above from: UniProt accession Q9BZZ5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8539 Human
    • Entrez Gene: 11800 Mouse
    • Omim: 609774 Human
    • SwissProt: Q9BZZ5 Human
    • SwissProt: O35841 Mouse
    • Unigene: 435771 Human
    • Unigene: 181824 Mouse
    • Unigene: 402557 Mouse
    • Alternative names

      • AAC 11 antibody
      • AAC-11 antibody
      • AAC11 antibody
      • Antiapoptosis clone 11 protein antibody
      • API 5 antibody
      • API-5 antibody
      • api5 antibody
      • API5 like 1 antibody
      • API5_HUMAN antibody
      • API5L1 antibody
      • Apoptosis inhibitor 5 antibody
      • Cell migration inducing gene 8 protein antibody
      • Cell migration-inducing gene 8 protein antibody
      • Fibroblast growth factor 2 interacting factor 2 antibody
      • Fibroblast growth factor 2-interacting factor antibody
      • FIF antibody
      • MIG 8 antibody
      • MIG8 antibody
      • Migration inducing protein MIG8 antibody
      • Protein XAGL antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of rat intestine tissue staining FIF using ab65836 at 1 μg/ml.

      Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65836 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

    • Western blot - Anti-FIF antibody (ab65836)
      Western blot - Anti-FIF antibody (ab65836)
      All lanes : Anti-FIF antibody (ab65836) at 0.5 µg/ml

      Lane 1 : Rat Cardiac Muscle Tissue Lysate
      Lane 2 : Rat Brain Tissue Lysate
      Lane 3 : Rat Testis Tissue Lysate
      Lane 4 : Rat Placenta Tissue Lysate
      Lane 5 : MCF-7 Cell Lysate
      Lane 6 : HeLa Cell Lysate
      Lane 7 : CEM Cell Lysate
      Lane 8 : SMMC Cell Lysate
      Lane 9 : COLO320 Cell Lysate

      Predicted band size: 58 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse intestine tissue staining FIF using ab65836 at 1 μg/ml.

      Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65836 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human placenta tissue staining FIF using ab65836 at 1 μg/ml.

      Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65836 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

    • Immunocytochemistry/ Immunofluorescence - Anti-FIF antibody (ab65836)
      Immunocytochemistry/ Immunofluorescence - Anti-FIF antibody (ab65836)

      Immunocytochemistry/ Immunofluorescence of HeLa cells staining FIF using ab65836 at 1 μg/ml.

    • Immunohistochemistry (Frozen sections) - Anti-FIF antibody (ab65836)
      Immunohistochemistry (Frozen sections) - Anti-FIF antibody (ab65836)

      Immunohistochemistry (Frozen sections) of Rat cardiac muscel tissue staining FIF using ab65836 at 1 μg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Rat brain tissue staining FIF using ab65836 at 1 μg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)
      This image shows human epidermal carcinoma tissue stained with ab65836 at 0.5-1µg/ml.
    • Immunocytochemistry/ Immunofluorescence - Anti-FIF antibody (ab65836)
      Immunocytochemistry/ Immunofluorescence - Anti-FIF antibody (ab65836)
      ICC/IF image of ab65836 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65836, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab65836? Please let us know so that we can cite the reference in this datasheet.

    ab65836 has been referenced in 3 publications.

    • Lu W  et al. Long Noncoding RNA-CERNA1 Stabilized Atherosclerotic Plaques in apolipoprotein E-/- Mice. J Cardiovasc Transl Res N/A:N/A (2019). PubMed: 30888631
    • Bousquet G  et al. High expression of apoptosis protein (Api-5) in chemoresistant triple-negative breast cancers: an innovative target. Oncotarget 10:6577-6588 (2019). PubMed: 31762939
    • Lu W  et al. Long Noncoding RNA LOC100129973 Suppresses Apoptosis by Targeting miR-4707-5p and miR-4767 in Vascular Endothelial Cells. Sci Rep 6:21620 (2016). WB . PubMed: 26887505

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-FIF antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (retina)
    Gel Running Conditions
    Reduced Denaturing (4-20)
    Loading amount
    30 µg
    Specification
    retina
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 08 2008

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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