Anti-FIF antibody (ab65836)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-FIF antibody
See all FIF primary antibodies -
Description
Rabbit polyclonal to FIF -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human FIF.
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Positive control
- WB: Rat Cardiac Muscle, Rat Brain, Rat Testis, Rat Placenta, MCF-7, HeLa, CEM, SMMC, and COLO320 Lysates. IHC-P: Rat Brain, Human placenta, Rat intestines, and Mouse intestines testis. IHC-F: Rat Cardiac Muscle. ICC/IF: HeLa cells.
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General notes
Previously labelled as Apoptosis inhibitor 5.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate, 2.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab65836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 0.5 - 1 µg/ml. | |
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). | |
| IHC-Fr | Use a concentration of 0.5 - 1 µg/ml. |
Target
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Function
Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. -
Tissue specificity
Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer. -
Sequence similarities
Belongs to the API5 family. -
Domain
Two regions, an N-terminal (aa 96-107) and a C-terminal (aa 274-311) are required for binding FGF2. -
Cellular localization
Cytoplasm and Nucleus. Cytoplasm. Mainly nuclear. Can also be cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 8539 Human
- Entrez Gene: 11800 Mouse
- Omim: 609774 Human
- SwissProt: Q9BZZ5 Human
- SwissProt: O35841 Mouse
- Unigene: 435771 Human
- Unigene: 181824 Mouse
- Unigene: 402557 Mouse
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Alternative names
- AAC 11 antibody
- AAC-11 antibody
- AAC11 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of rat intestine tissue staining FIF using ab65836 at 1 μg/ml.
Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65836 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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Western blot - Anti-FIF antibody (ab65836)All lanes : Anti-FIF antibody (ab65836) at 0.5 µg/ml
Lane 1 : Rat Cardiac Muscle Tissue Lysate
Lane 2 : Rat Brain Tissue Lysate
Lane 3 : Rat Testis Tissue Lysate
Lane 4 : Rat Placenta Tissue Lysate
Lane 5 : MCF-7 Cell Lysate
Lane 6 : HeLa Cell Lysate
Lane 7 : CEM Cell Lysate
Lane 8 : SMMC Cell Lysate
Lane 9 : COLO320 Cell Lysate
Predicted band size: 58 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse intestine tissue staining FIF using ab65836 at 1 μg/ml.
Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65836 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human placenta tissue staining FIF using ab65836 at 1 μg/ml.
Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65836 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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Immunocytochemistry/ Immunofluorescence - Anti-FIF antibody (ab65836)Immunocytochemistry/ Immunofluorescence of HeLa cells staining FIF using ab65836 at 1 μg/ml.
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Immunohistochemistry (Frozen sections) - Anti-FIF antibody (ab65836)Immunohistochemistry (Frozen sections) of Rat cardiac muscel tissue staining FIF using ab65836 at 1 μg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Rat brain tissue staining FIF using ab65836 at 1 μg/ml.
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Western blot - Anti-FIF antibody (ab65836)Anti-FIF antibody (ab65836) at 2 µg/ml + rat cardiac muscle tissue lysate
Predicted band size: 58 kDa
Observed band size: 58 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)This image shows human epidermal carcinoma tissue stained with ab65836 at 0.5-1µg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-FIF antibody (ab65836)ICC/IF image of ab65836 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65836, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
This product has been referenced in:
- Lu W et al. Long Noncoding RNA-CERNA1 Stabilized Atherosclerotic Plaques in apolipoprotein E-/- Mice. J Cardiovasc Transl Res N/A:N/A (2019). Read more (PubMed: 30888631) »
- Lu W et al. Long Noncoding RNA LOC100129973 Suppresses Apoptosis by Targeting miR-4707-5p and miR-4767 in Vascular Endothelial Cells. Sci Rep 6:21620 (2016). WB . Read more (PubMed: 26887505) »
