• Product name

  • Description

    Rabbit polyclonal to FIG4
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human FIG4 aa 378-476.


    Database link: Q92562

  • Positive control

    • WB: U-251 MG cell lysate. ICC/IF: SK-MEL-30 cells.



Our Abpromise guarantee covers the use of ab221758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250. Predicted molecular weight: 103 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.


  • Function

    The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2). In vitro, hydrolyzes all three D5-phosphorylated polyphosphoinositide substrates in the order PtdIns(4,5)P2 > PtdIns(3,5)P2 > PtdIns(3,4,5)P3. Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.
  • Involvement in disease

    Defects in FIG4 are the cause of Charcot-Marie-Tooth disease type 4J (CMT4J) [MIM:611228]. CMT4J is a recessive demyelinating, severe form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies characterized by severely reduced motor nerve conduction velocities (NCVs) (less than 38m/s) and segmental demyelination and remyelination, and primary peripheral axonal neuropathies characterized by normal or mildly reduced NCVs and chronic axonal degeneration and regeneration on nerve biopsy.
    Defects in FIG4 are the cause of amyotrophic lateral sclerosis type 11 (ALS11) [MIM:612577]. ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10%.
  • Sequence similarities

    Contains 1 SAC domain.
  • Cellular localization

    Endosome membrane. Localization requires VAC14 and PIKFYVE.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5-bisphosphate 5-phosphatase antibody
    • ALS 11 antibody
    • ALS11 antibody
    • BTOP antibody
    • CMT4J antibody
    • dJ249I4. antibody
    • Fig4 antibody
    • FIG4 homolog (S cerevisiae) antibody
    • FIG4 homolog antibody
    • FIG4 homolog SAC domain containing lipid phosphatase antibody
    • FIG4 homolog SAC1 lipid phosphatase domain containing antibody
    • FIG4 homolog, SAC1 lipid phosphatase domain containing (S cerevisiae antibody
    • FIG4 phosphoinositide 5 phosphatase antibody
    • FIG4_HUMAN antibody
    • KIAA0274 antibody
    • phosphatidylinositol 3 5 bisphosphate 5 phosphatase antibody
    • Phosphatidylinositol 3 antibody
    • Phosphatidylinositol 3,5 bisphosphate 5 phosphatase antibody
    • Polyphosphoinositide phosphatase antibody
    • RP1-249I4.1 antibody
    • SAC 3 antibody
    • Sac domain containing inositol phosphatase 3 antibody
    • SAC domain containing protein 3 antibody
    • SAC domain-containing protein 3 antibody
    • SAC3 antibody
    • YVS antibody
    see all


  • PFA-fixed, Triton X-100 permeabilized SK-MEL-30 cells stained for FIG4 (green) using ab221758 at 4 µg/ml in ICC/IF.

  • All lanes : Anti-FIG4 antibody (ab221758) at 1/100 dilution

    Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
    Lane 2 : U-251 MG (human brain glioma cell line) cell lysate

    Predicted band size: 103 kDa


ab221758 has not yet been referenced specifically in any publications.

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