Key features and details
- Rabbit polyclonal to FIGNL1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FIGNL1 antibody
See all FIGNL1 primary antibodies
DescriptionRabbit polyclonal to FIGNL1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide, corresponding to a region between amino acids 1-50 of Human FIGNL1 (NP_071399.2)
- Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 71 kDa (predicted molecular weight: 74 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceMay regulate osteoblast proliferation and differentiation.
- Fidgetin like protein 1 antibody
All lanes : Anti-FIGNL1 antibody (ab70841) at 1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 74 kDa
Observed band size: 71 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Detection of Human FIGNL1 by Immunoprecipitation in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded), using ab70841 for IP at 3 µg/mg lysate. Subsequent WB detection was performed using 1 µg/mg ab70841 and chemiluminescence with an exposure time of 30 seconds.
ab70841 has not yet been referenced specifically in any publications.