Anti-Filaggrin antibody (ab81468)
Key features and details
- Rabbit polyclonal to Filaggrin
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Filaggrin antibody
See all Filaggrin primary antibodies -
Description
Rabbit polyclonal to Filaggrin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Filaggrin (N terminal).
Database link: P20930 -
General notes
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 23rd June 2017. The following lots are still unpurified and still in stock as of 23rd June 2017 - GR309720-5, GR3178284-1, GR309720-6, GR309720-2, GR309720-3, GR309720-4. Lot numbers other than GR309720-5, GR3178284-1, GR309720-6, GR309720-2, GR309720-3, GR309720-4 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
|
Notes |
---|
ICC/IF
Use at an assay dependent concentration. |
Target
-
Function
Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. -
Tissue specificity
Keratohyalin granules. -
Involvement in disease
Ichthyosis vulgaris
Dermatitis atopic 2 -
Sequence similarities
Belongs to the S100-fused protein family.
Contains 2 EF-hand domains.
Contains 23 filaggrin repeats. -
Post-translational
modificationsFilaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate.
Undergoes deimination of some arginine residues (citrullination). - Information by UniProt
-
Database links
- Entrez Gene: 2312 Human
- Omim: 135940 Human
- SwissProt: P20930 Human
- Unigene: 654510 Human
-
Alternative names
- ATOD2 antibody
- Epidermal filaggrin antibody
- FILA_HUMAN antibody
see all
Images
-
ICC/IF image of ab81468 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81468, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (26)
ab81468 has been referenced in 26 publications.
- Skieresz-Szewczyk K et al. Alpha-Keratin, Keratin-Associated Proteins and Transglutaminase 1 Are Present in the Ortho- and Parakeratinized Epithelium of the Avian Tongue. Cells 11:N/A (2022). PubMed: 35741029
- Ali G & Abdelalim EM Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells. STAR Protoc 3:101613 (2022). PubMed: 35990735
- Hao L et al. Repurposing the anthelmintic praziquantel to treat psoriasis. Br J Pharmacol 178:4726-4740 (2021). PubMed: 34363611
- Wang X et al. FAK Shutdown: Consequences on Epithelial Morphogenesis and Biomarker Expression Involving an Innovative Biomaterial for Tissue Regeneration. Int J Mol Sci 22:N/A (2021). PubMed: 34575938
- Bize C et al. Barrier disruption, dehydration and inflammation: Investigation of the vicious circle underlying dry skin. Int J Cosmet Sci 43:729-737 (2021). PubMed: 34716992