Anti-Filaggrin antibody [FLG/1561] (ab218395)
Key features and details
- Mouse monoclonal [FLG/1561] to Filaggrin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Filaggrin antibody [FLG/1561]
See all Filaggrin primary antibodies -
Description
Mouse monoclonal [FLG/1561] to Filaggrin -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Filaggrin aa 998-1104. The exact sequence is proprietary.
Database link: P20930 -
Positive control
- Human skin tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab218395 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
FLG/1561 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
(Primary incubation for 30 minutes at RT). |
Notes |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (Primary incubation for 30 minutes at RT). |
Target
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Function
Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. -
Tissue specificity
Keratohyalin granules. -
Involvement in disease
Ichthyosis vulgaris
Dermatitis atopic 2 -
Sequence similarities
Belongs to the S100-fused protein family.
Contains 2 EF-hand domains.
Contains 23 filaggrin repeats. -
Post-translational
modificationsFilaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate.
Undergoes deimination of some arginine residues (citrullination). - Information by UniProt
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Database links
- Entrez Gene: 2312 Human
- Omim: 135940 Human
- SwissProt: P20930 Human
- Unigene: 654510 Human
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Alternative names
- ATOD2 antibody
- Epidermal filaggrin antibody
- FILA_HUMAN antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab218395 has been referenced in 5 publications.
- Cadau S et al. An Inflamed and Infected Reconstructed Human Epidermis to Study Atopic Dermatitis and Skin Care Ingredients. Int J Mol Sci 23:N/A (2022). PubMed: 36361668
- Rahim AB et al. The Polyamine Regulator AMD1 Upregulates Spermine Levels to Drive Epidermal Differentiation. J Invest Dermatol 141:2178-2188.e6 (2021). PubMed: 33984347
- Zhou J et al. Transcriptomic Analysis of Healthy and Atopic Dermatitis Samples Reveals the Role of IL-37 in Human Skin. Immunohorizons 5:830-843 (2021). PubMed: 34702760
- Baltazar T et al. Three Dimensional Bioprinting of a Vascularized and Perfusable Skin Graft Using Human Keratinocytes, Fibroblasts, Pericytes, and Endothelial Cells. Tissue Eng Part A 26:227-238 (2020). PubMed: 31672103
- Tan CL et al. Nicotinamide Metabolism Modulates the Proliferation/Differentiation Balance and Senescence of Human Primary Keratinocytes. J Invest Dermatol 139:1638-1647.e3 (2019). PubMed: 30776433