Overview

  • Product name

    Anti-Filaggrin antibody [FLG/1561]
    See all Filaggrin primary antibodies
  • Description

    Mouse monoclonal [FLG/1561] to Filaggrin
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Filaggrin aa 998-1104. The exact sequence is proprietary.
    Database link: P20930

  • Positive control

    • Human skin tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab218395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(Primary incubation for 30 minutes at RT).

Target

  • Function

    Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
  • Tissue specificity

    Keratohyalin granules.
  • Involvement in disease

    Ichthyosis vulgaris
    Dermatitis atopic 2
  • Sequence similarities

    Belongs to the S100-fused protein family.
    Contains 2 EF-hand domains.
    Contains 23 filaggrin repeats.
  • Post-translational
    modifications

    Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate.
    Undergoes deimination of some arginine residues (citrullination).
  • Information by UniProt
  • Database links

  • Alternative names

    • ATOD2 antibody
    • Epidermal filaggrin antibody
    • FILA_HUMAN antibody
    • Filaggrin antibody
    • Filaggrin precursor antibody
    • Fillagrin antibody
    • FLG antibody
    • Profilaggrin antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skin tissue labeling Filaggrin with ab218395 at 1 µg/ml.

References

ab218395 has not yet been referenced specifically in any publications.

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