Anti-Filaggrin antibody [FLG/1562] - BSA and Azide free (ab218864)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filaggrin antibody [FLG/1562] - BSA and Azide free (ab218864)

    Key features and details

    • Mouse monoclonal [FLG/1562] to Filaggrin - BSA and Azide free
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    Overview

    • Product name

      Anti-Filaggrin antibody [FLG/1562] - BSA and Azide free
      See all Filaggrin primary antibodies

    • Description

      Mouse monoclonal [FLG/1562] to Filaggrin - BSA and Azide free

    • Host species

      Mouse

    • Tested applications

      Suitable for: IHC-Pmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Recombinant fragment within Human Filaggrin aa 998-1104. The exact sequence is proprietary.
      Database link: P20930

    • Positive control

      • Human skin tissue.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab218864 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    (Primary incubation for 30 min at RT).

    Target

    • Function

      Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.

    • Tissue specificity

      Keratohyalin granules.

    • Involvement in disease

      Ichthyosis vulgaris
      Dermatitis atopic 2

    • Sequence similarities

      Belongs to the S100-fused protein family.
      Contains 2 EF-hand domains.
      Contains 23 filaggrin repeats.

    • Post-translational
      modifications

      Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate.
      Undergoes deimination of some arginine residues (citrullination).
    • Information by UniProt

    • Database links

      • Alternative names

        • ATOD2 antibody
        • Epidermal filaggrin antibody
        • FILA_HUMAN antibody
        • Filaggrin antibody
        • Filaggrin precursor antibody
        • Fillagrin antibody
        • FLG antibody
        • Profilaggrin antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filaggrin antibody [FLG/1562] - BSA and Azide free (ab218864)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filaggrin antibody [FLG/1562] - BSA and Azide free (ab218864)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skin tissue labeling Filaggrin with ab218864 at 1 µg/ml.

      References (0)

      Publishing research using ab218864? Please let us know so that we can cite the reference in this datasheet.

      ab218864 has not yet been referenced specifically in any publications.

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