For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    filaggrin-antibody-flg1562-bsa-and-azide-free-ab218864.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Tags & Cell Markers Cell Type Markers Epi / Endo-thelial
Share by email

Anti-Filaggrin antibody [FLG/1562] - BSA and Azide free (ab218864)

  • Datasheet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filaggrin antibody [FLG/1562] - BSA and Azide free (ab218864)

    Key features and details

    • Mouse monoclonal [FLG/1562] to Filaggrin - BSA and Azide free
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Protein
    Product image
    Recombinant Human Filaggrin protein (His tag) (ab240777)

    View more associated products

    Overview

    • Product name

      Anti-Filaggrin antibody [FLG/1562] - BSA and Azide free
      See all Filaggrin primary antibodies
    • Description

      Mouse monoclonal [FLG/1562] to Filaggrin - BSA and Azide free
    • Host species

      Mouse
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant fragment within Human Filaggrin aa 998-1104. The exact sequence is proprietary.
      Database link: P20930

    • Positive control

      • Human skin tissue.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      Constituent: 100% PBS
    • Carrier free

      Yes
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      ab218864 was purified from Bioreactor Concentrate by Protein A/G.
    • Clonality

      Monoclonal
    • Clone number

      FLG/1562
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Tags & Cell Markers
      • Cell Type Markers
      • Epi / Endo-thelial
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Proteins
      • Cytokeratin
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Intermediate Filaments
      • Class I
      • Cytokeratins

    Associated products

    • Alternative Versions

      • Anti-Filaggrin antibody [FLG/1562] (ab218397)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human Filaggrin protein (His tag) (ab240777)

    Applications

    Our Abpromise guarantee covers the use of ab218864 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    (Primary incubation for 30 min at RT).

    Target

    • Function

      Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
    • Tissue specificity

      Keratohyalin granules.
    • Involvement in disease

      Ichthyosis vulgaris
      Dermatitis atopic 2
    • Sequence similarities

      Belongs to the S100-fused protein family.
      Contains 2 EF-hand domains.
      Contains 23 filaggrin repeats.
    • Post-translational
      modifications

      Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate.
      Undergoes deimination of some arginine residues (citrullination).
    • Target information above from: UniProt accession P20930 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2312 Human
      • Omim: 135940 Human
      • SwissProt: P20930 Human
      • Unigene: 654510 Human
      • Alternative names

        • ATOD2 antibody
        • Epidermal filaggrin antibody
        • FILA_HUMAN antibody
        • Filaggrin antibody
        • Filaggrin precursor antibody
        • Fillagrin antibody
        • FLG antibody
        • Profilaggrin antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filaggrin antibody [FLG/1562] - BSA and Azide free (ab218864)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filaggrin antibody [FLG/1562] - BSA and Azide free (ab218864)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skin tissue labeling Filaggrin with ab218864 at 1 µg/ml.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab218864? Please let us know so that we can cite the reference in this datasheet.

      ab218864 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab218864.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.