Anti-Filaggrin antibody [FLG/1563] (ab237996)
Key features and details
- Mouse monoclonal [FLG/1563] to Filaggrin
- Suitable for: Protein Array, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Anti-Filaggrin antibody [FLG/1563]
See all Filaggrin primary antibodies -
Description
Mouse monoclonal [FLG/1563] to Filaggrin -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Filaggrin aa 998-1104. The exact sequence is proprietary.
Database link: P20930 -
Positive control
- IHC-P: Human skin tissue.
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
FLG/1563 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Protein Array |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml.
Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperaturefor 20 minutes. Incubate with primary antibody for 30 minutes at room temperature. |
Notes |
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Protein Array
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperaturefor 20 minutes. Incubate with primary antibody for 30 minutes at room temperature. |
Target
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Function
Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. -
Tissue specificity
Keratohyalin granules. -
Involvement in disease
Ichthyosis vulgaris
Dermatitis atopic 2 -
Sequence similarities
Belongs to the S100-fused protein family.
Contains 2 EF-hand domains.
Contains 23 filaggrin repeats. -
Post-translational
modificationsFilaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate.
Undergoes deimination of some arginine residues (citrullination). - Information by UniProt
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Database links
- Entrez Gene: 2312 Human
- Omim: 135940 Human
- SwissProt: P20930 Human
- Unigene: 654510 Human
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Alternative names
- ATOD2 antibody
- Epidermal filaggrin antibody
- FILA_HUMAN antibody
see all
Images
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ab237996 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. -
Formalin-fixed, paraffin-embedded human skin tissue stained for Filaggrin with ab237996 at 1 µg/ml in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab237996 has not yet been referenced specifically in any publications.