Overview

  • Product name

    Anti-Filaggrin antibody [FLG/1563] - BSA and Azide free
    See all Filaggrin primary antibodies
  • Description

    Mouse monoclonal [FLG/1563] to Filaggrin - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: Protein Array, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Filaggrin aa 998-1104. The exact sequence is proprietary.
    Database link: P20930

  • Positive control

    • IHC-P: Human skin tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab237839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array Use at an assay dependent concentration.
IHC-P Use a concentration of 0.5 - 1 µg/ml.

Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperaturefor 20 minutes. Incubate with primary antibody for 30 minutes at room temperature.

Target

  • Function

    Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
  • Tissue specificity

    Keratohyalin granules.
  • Involvement in disease

    Ichthyosis vulgaris
    Dermatitis atopic 2
  • Sequence similarities

    Belongs to the S100-fused protein family.
    Contains 2 EF-hand domains.
    Contains 23 filaggrin repeats.
  • Post-translational
    modifications

    Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate.
    Undergoes deimination of some arginine residues (citrullination).
  • Information by UniProt
  • Database links

  • Alternative names

    • ATOD2 antibody
    • Epidermal filaggrin antibody
    • FILA_HUMAN antibody
    • Filaggrin antibody
    • Filaggrin precursor antibody
    • Fillagrin antibody
    • FLG antibody
    • Profilaggrin antibody
    see all

Images

  • This data was produced with ab237996, the same antibody in a different formulation with BSA and Azide.
    ab237996 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
  • Formalin-fixed, paraffin-embedded human skin tissue stained for Filaggrin with ab237839 at 1 µg/ml in immunohistochemical analysis.

References

ab237839 has not yet been referenced specifically in any publications.

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