Overview

  • Product name

    Anti-Filaggrin antibody [FLG/1957R]
    See all Filaggrin primary antibodies
  • Description

    Rabbit monoclonal [FLG/1957R] to Filaggrin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Filaggrin aa 198-288. The exact sequence is proprietary.
    Database link: P20930

  • Positive control

    • IHC-P: Human skin tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab234406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml.

Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 minutes at room temperature.

Target

  • Function

    Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
  • Tissue specificity

    Keratohyalin granules.
  • Involvement in disease

    Ichthyosis vulgaris
    Dermatitis atopic 2
  • Sequence similarities

    Belongs to the S100-fused protein family.
    Contains 2 EF-hand domains.
    Contains 23 filaggrin repeats.
  • Post-translational
    modifications

    Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate.
    Undergoes deimination of some arginine residues (citrullination).
  • Information by UniProt
  • Database links

  • Alternative names

    • ATOD2 antibody
    • Epidermal filaggrin antibody
    • FILA_HUMAN antibody
    • Filaggrin antibody
    • Filaggrin precursor antibody
    • Fillagrin antibody
    • FLG antibody
    • Profilaggrin antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human skin tissue stained for Filaggrin using ab234406 at 1 μg/ml in immunohistochemical analysis.

  • SDS-PAGE - Anti-Filaggrin antibody [FLG/1957R] (ab234406) at 2.5 µg.
    Lane 1: Non-reduced.
    Lane 2: Reduced.

References

ab234406 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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