Recombinant Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] (ab75978)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2310AY] to Filamin A (phospho S2152) + Filamin B (phospho S2107)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY]
See all Filamin A (phospho S2152) + Filamin B (phospho S2107) primary antibodies -
Description
Rabbit monoclonal [EP2310AY] to Filamin A (phospho S2152) + Filamin B (phospho S2107) -
Host species
Rabbit -
Specificity
ab75978 detects Filamin A phosphorylated on serine 2152 and Filamin B phosphorylated on serine 2107.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293. NIH/3T3, and C6 whole cell lysates. IHC-P: Human, Mouse, and Rat kidney tissue.
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General notes
This product has switched from a hybridoma to recombinant production method on 9th June 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2310AY -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100000. Detects a band of approximately 281 kDa (predicted molecular weight: 281 kDa).
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IHC-P |
1/1200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/100000. Detects a band of approximately 281 kDa (predicted molecular weight: 281 kDa). |
IHC-P
1/1200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Relevance
Filamin A-P21333. Function: Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking. Involved in ciliogenesis. Filamin B-O75369. Function: Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro. -
Database links
- Entrez Gene: 2316 Human
- Entrez Gene: 2317 Human
- Entrez Gene: 192176 Mouse
- Entrez Gene: 286940 Mouse
- Entrez Gene: 293860 Rat
- Entrez Gene: 306204 Rat
- SwissProt: O75369 Human
- SwissProt: P21333 Human
see all -
Alternative names
- ABP-278 antibody
- ABP-280 antibody
- ABP-280 homolog antibody
see all
Images
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All lanes : Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] (ab75978) at 1/100000 dilution
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate
Lane 3 : HEK-293 (Human embryonic kidney epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate, then the membrane was incubated with Alkaline phosphatase
Lysates/proteins at 15 µg per lane.
Predicted band size: 281 kDa
Exposure time: 10 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] (ab75978) at 1/100000 dilution
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 3 : Calyculin A for 30 minutes whole cell lysate, then the membrane was incubated with Alkaline phosphatase
Lysates/proteins at 15 µg per lane.
Predicted band size: 281 kDa
Observed band size: 280 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] (ab75978) at 1/100000 dilution
Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 2 : C6 (Rat glial tumor glial cell) treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 3 : C6 (Rat glial tumor glial cell) treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane was incubated with Alkaline phosphatase
Lysates/proteins at 15 µg per lane.
Predicted band size: 281 kDa
Observed band size: 280 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Filamin A (phospho S2152) + Filamin B (phospho S2107) with ab75978 at 1/1200 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Human kidney tissue without alkaline phosphatase treatment (A) compared to no signal detected when treated with alkaline phosphatase (B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling Filamin A (phospho S2152) + Filamin B (phospho S2107) with ab75978 at 1/1200 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Mouse kidney tissue without alkaline phosphatase treatment (A) compared to no signal detected when treated with alkaline phosphatase (B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labelling Filamin A (phospho S2152) + Filamin B (phospho S2107) with ab75978 at 1/1200 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Rat kidney tissue without alkaline phosphatase treatment (A) compared to no signal detected when treated with alkaline phosphatase (B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab75978 has been referenced in 2 publications.
- Allam AB et al. Ureaplasma parvum infection alters filamin A dynamics in host cells. BMC Infect Dis 11:101 (2011). ICC/IF, ELISA ; Rat . PubMed: 21507248
- Bogatcheva NV et al. Molecular mechanisms mediating protective effect of cAMP on lipopolysaccharide (LPS)-induced human lung microvascular endothelial cells (HLMVEC) hyperpermeability. J Cell Physiol 221:750-9 (2009). WB ; Human . PubMed: 19725051