Product nameAnti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY]
DescriptionRabbit monoclonal [EP2310AY] to Filamin A (phospho S2152) + Filamin B (phospho S2107)
Specificityab75978 detects Filamin A phosphorylated on serine 2152 and Filamin B phosphorylated on serine 2107.
Tested applicationsSuitable for: ICC/IF, ELISA, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Filamin A (phospho S2152) + Filamin B (phospho S2107) aa 2100-2200.
Database link: P21333
- HeLa cell lysates, untreated or treated with calyculin A; human uterus tissue.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab75978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/200.|
|ELISA||Use at an assay dependent concentration. PubMed: 21507248|
|WB||1/10000 - 1/20000. Detects a band of approximately 281 kDa (predicted molecular weight: 281 kDa).|
|IHC-P||1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
RelevanceFilamin A-P21333. Function: Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking. Involved in ciliogenesis. Filamin B-O75369. Function: Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro.
- ABP-278 antibody
- ABP-280 antibody
- ABP-280 homolog antibody
All lanes : Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] (ab75978) at 1/20000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 5nM calyculin A for 2 hours whole cell lysate with 5% NFDM/TBST
Lane 2 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 281 kDa
Observed band size: 281 kDa
Exposure time: 180 seconds
Immunofluorescence analysis of U. parvum infected BPH-1 (Human benign prostate) cells staining Filamin A (phospho S2152) and Filamin B (phospho S2107) with ab75978 (red). Cells were fixed with formaldehyde before incubating with primary antibody. An AlexaFluor®594-conjugated goat anti-rabbit IgG was used as the secondary antibody.
ab75978 at 1/100 dilution staining Filamin A (phospho S2152) and Filamin B (phospho S2107) in human uterus by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This product has been referenced in:
- Allam AB et al. Ureaplasma parvum infection alters filamin A dynamics in host cells. BMC Infect Dis 11:101 (2011). ICC/IF, ELISA ; Rat . Read more (PubMed: 21507248) »
- Bogatcheva NV et al. Molecular mechanisms mediating protective effect of cAMP on lipopolysaccharide (LPS)-induced human lung microvascular endothelial cells (HLMVEC) hyperpermeability. J Cell Physiol 221:750-9 (2009). WB ; Human . Read more (PubMed: 19725051) »