Recombinant Anti-Filamin C antibody [EPR14498(B)] (ab180941)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14498(B)] to Filamin C
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Filamin C antibody [EPR14498(B)]
See all Filamin C primary antibodies -
Description
Rabbit monoclonal [EPR14498(B)] to Filamin C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human skeletal muscle and fetal heart lysates; Human muscle tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14498(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 291 kDa (predicted molecular weight: 291 kDa).
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IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 291 kDa (predicted molecular weight: 291 kDa). |
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Relevance
FLNC is a muscle-specific filamin, which plays a central role in muscle cells, probably by functioning as a large actin-cross-linking protein. May be involved in reorganizing the actin cytoskeleton in response to signaling events, and may also display structural functions at the Z-disks in muscle cells. Defects in FLNC are the cause of autosomal dominant filaminopathy. Myofibrillar myopathy (MFM) is a neuromuscular disorder, usually with an adult onset, characterized by focal myofibrillar destruction and pathological cytoplasmic protein aggregations. Autosomal dominant filaminopathy is a form of MFM characterized by morphological features of MFM and clinical features of a limb-girdle myopathy. A heterozygous nonsense mutation which segregates with the disease, has been identified in the FLNC gene. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 2318 Human
- SwissProt: Q14315 Human
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Alternative names
- ABPL antibody
- Actin binding like protein antibody
- FIlamin 2 antibody
see all
Images
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All lanes : Anti-Filamin C antibody [EPR14498(B)] (ab180941) at 1/50000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 291 kDa
Observed band size: 291 kDa -
Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling Filamin C with ab180941 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (6)
ab180941 has been referenced in 6 publications.
- Li Z et al. Hgs Deficiency Caused Restrictive Cardiomyopathy via Disrupting Proteostasis. Int J Biol Sci 18:2018-2031 (2022). PubMed: 35342336
- Chillappagari S et al. Therapeutic induction of Bcl2-associated athanogene 3-mediated autophagy in idiopathic pulmonary fibrosis. Clin Transl Med 12:e935 (2022). PubMed: 35834635
- Agarwal R et al. Filamin C Cardiomyopathy Variants Cause Protein and Lysosome Accumulation. Circ Res 129:751-766 (2021). PubMed: 34405687
- Chen Y et al. Downregulation of Filamin a Expression in the Aorta Is Correlated With Aortic Dissection. Front Cardiovasc Med 8:690846 (2021). PubMed: 34485398
- Zhang R et al. Quantitative phosphoproteomic analysis reveals chemoresistance-related proteins and signaling pathways induced by rhIL-6 in human osteosarcoma cells. Cancer Cell Int 21:581 (2021). PubMed: 34717622
- Papizan JB et al. Deficiency in Kelch protein Klhl31 causes congenital myopathy in mice. J Clin Invest 127:3730-3740 (2017). PubMed: 28872460