Overview

  • Product name

  • Description

    Rabbit polyclonal to FILGAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human FILGAP aa 630-680 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    SETFVGNSSSN HSALHSLVSS LKQEMTKQKI EYESRIKSLE QRNLTLETEM


    Database link: Q8N264

  • Positive control

    • Human lung carcinoma tissue; Rat kidney lysate; Human colon carcinoma lysate.
  • General notes

     This product was previously labelled as Rho GTPase-activating protein 24

     

Properties

Applications

Our Abpromise guarantee covers the use of ab203874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
WB 1/100 - 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).

Target

  • Relevance

    ARHGAP24 is a rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. It acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. It controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. There are five different isoforms.Isoform 1 is widely expressed with a higher level in kidney. Isoform 2 is mainly expressed in endothelial cells and is involved in modulation of angiogenesis.
  • Cellular localization

    Cytoplasmic, cytoskeleton. Cell junction: adherens junction, focal adhesion. Cell projection.
  • Database links

  • Alternative names

    • ARHGAP24 antibody
    • DKFZP564B1162 antibody
    • Filamin A associated RhoGAP antibody
    • FILGAP antibody
    • FLJ33877 antibody
    • p73 antibody
    • p73RhoGAP antibody
    • RAC1 and CDC4 specific GTPase activating protein of 72kDa antibody
    • RCGAP72 antibody
    • Rho GTPase activating protein 24 antibody
    • Rho type GTPase activating protein 24 antibody
    • RhoGAP of 73 kDa antibody
    • Sarcoma antigen NY SAR 88 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling FILGAP with ab203874 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

  • All lanes : Anti-FILGAP antibody (ab203874) at 1/200 dilution

    Lane 1 : Human colon carcinoma lysate
    Lane 2 : Rat kidney lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution

    Predicted band size: 84 kDa

References

This product has been referenced in:

  • Wang L  et al. Rho GTPase Activating Protein 24 (ARHGAP24) Silencing Promotes Lung Cancer Cell Migration and Invasion by Activating ß-Catenin Signaling. Med Sci Monit 25:21-31 (2019). Read more (PubMed: 30599132) »
  • Zhang S  et al. ARHGAP24 regulates cell ability and apoptosis of colorectal cancer cells via the regulation of P53. Oncol Lett 16:3517-3524 (2018). Read more (PubMed: 30127956) »
See all 3 Publications for this product

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