Key features and details
- Rabbit polyclonal to FILIP1L
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-FILIP1L antibody
See all FILIP1L primary antibodies
DescriptionRabbit polyclonal to FILIP1L
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human FILIP1L aa 1-200.
Database link: Q4L180
- WB: Rat stomach lysate; Mouse kidney lysate. IHC-P: Human colon cancer and adrenal gland tissues. ICC/IF: HepG2 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 130 kDa.|
|IHC-P||1/100 - 1/500.|
|ICC/IF||1/50 - 1/500.|
FunctionActs as a regulator of the antiangiogenic activity on endothelial cells. When overexpressed in endothelial cells, leads to inhibition of cell proliferation and migration and an increase in apoptosis. Inhibits melanoma growth When expressed in tumor-associated vasculature.
Tissue specificityExpressed in endothelial cells, colon and colon cancers. In the colon, expressed in the vasculature and muscularis mucosa. In colon cancer, strongly expressed in tumor stroma and the vasculature (at protein level). Expressed in ovarian epithelial cells. Down-regulated in ovarian cancer.
Sequence similaritiesBelongs to the FILIP1 family.
Cellular localizationCytoplasm. Membrane. Nucleus.
- Information by UniProt
- 130 kDa GPBP-interacting protein antibody
- 90 kDa GPBP-interacting protein antibody
- COL4A3BPIP antibody
Paraffin-embedded human colon cancer tissue stained for FILIP1L using ab236979 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-FILIP1L antibody (ab236979) at 1/500 dilution
Lane 1 : Rat stomach lysate
Lane 2 : Mouse kidney lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 130 kDa
Paraffin-embedded human adrenal gland tissue stained for FILIP1L using ab236979 at 1/100 dilution in immunohistochemical analysis.
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for FILIP1L (green) using ab236979 at 1/166 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236979 has not yet been referenced specifically in any publications.