Product nameAnti-FIP antibody
See all FIP primary antibodies
DescriptionRabbit polyclonal to FIP
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Orangutan
Synthetic peptide corresponding to Human FIP aa 296-346.
- Purchase matching WB positive control:Recombinant Human FIP protein
- HeLa, Jurkat and 293T whole cell lysates
Protein previously labeled as USF2.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab125184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 37 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionTranscription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
- Information by UniProt
- bHLHb12 antibody
- Class B basic helix-loop-helix protein 12 antibody
- FIP antibody
All lanes : Anti-FIP antibody (ab125184) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 30 seconds
ab125184, at 6 µg/mg lysate, detecting FIP in 293T cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
For the subsequent Western blot, ab125184 was used at 1 µg/ml.
The blot was developed using the ECL technique, with an exposure time of 10 seconds.