Overview

  • Product name
    Anti-FIP200 antibody
    See all FIP200 primary antibodies
  • Description
    Rabbit polyclonal to FIP200
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset, Orangutan
  • Immunogen

    Synthetic peptide within Human FIP200 aa 1544-1594 (C terminal). The exact sequence is proprietary. NP_055596.3
    Sequence:

    EGASGAS RRPWVLGKVM EKEYCQAKKA QNRFKVPLGT KFYRVKAVSW NKKV


    Database link: Q8TDY2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • HeLa, 293T and TCMK1 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab176816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 183 kDa.

Target

  • Function
    Implicated in the regulation of RB1 expression. Functions as a DNA-binding transcription factor. Is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. Required for autophagosome formation (By similarity). Inhibits PTK2/FAK1 and PTK2B/PYK2 activity and activation of downstream signaling pathways.
  • Tissue specificity
    Expression levels correlated closely with those of RB1 in cancer cell lines as well as in various normal human tissues. Abundantly expressed in human musculoskeletal and cultured osteosarcoma cells.
  • Developmental stage
    Expression was difficult to detect in immature proliferating chondroblasts or myogenic cells in embryos, but became obvious and prominent concomitantly with the maturation of osteocytes, chondrocytes, and skeletal muscle cells. Expression in these musculoskeletal cells increased with RB1 expression, which is linked to the terminal differentiation of many tissues and cells. The introduction of the wild-type protein decreased the formation of macroscopic colonies in a cell growth assay.
  • Cellular localization
    Nucleus. Cytoplasm > cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • FAK family kinase-interacting protein of 200 kDa antibody
    • FIP200 antibody
    • RB1-inducible coiled-coil protein 1 antibody
    • RB1CC1 antibody
    • RBCC1_HUMAN antibody
    see all

Images

  • Western blot - Anti-FIP200 antibody (ab176816)
    Western blot - Anti-FIP200 antibody (ab176816)
    All lanes : Anti-FIP200 antibody (ab176816) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysates at 50 µg
    Lane 2 : HeLa whole cell lysates at 15 µg
    Lane 3 : HeLa whole cell lysates at 5 µg
    Lane 4 : 293T whole cell lysates at 50 µg

    Developed using the ECL technique.

    Predicted band size: 183 kDa


    Exposure time: 30 seconds
  • Western blot - Anti-FIP200 antibody (ab176816)
    Western blot - Anti-FIP200 antibody (ab176816)
    All lanes : Anti-FIP200 antibody (ab176816) at 0.2 µg/ml

    Lane 1 : TCMK1 whole cell lysates at 50 µg
    Lane 2 : TCMK1 whole cell lysates at 15 µg

    Developed using the ECL technique.

    Predicted band size: 183 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-FIP200 antibody (ab176816)
    Immunoprecipitation - Anti-FIP200 antibody (ab176816)

    ab176816 at 0.1 µg/ml detecting FIP200 in HeLa whole cell lysate by WB following IP. 

    Lane 1: ab176816 at 3µg/mg of lysate
    Lane 2: Control IgG.

    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

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