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    fip200-antibody-ab176816.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Kinases FAK / PYK
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Anti-FIP200 antibody (ab176816)

  • Datasheet
  • SDS
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Western blot - Anti-FIP200 antibody (ab176816)
  • Western blot - Anti-FIP200 antibody (ab176816)
  • Immunoprecipitation - Anti-FIP200 antibody (ab176816)

Key features and details

  • Rabbit polyclonal to FIP200
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-FIP200 antibody
    See all FIP200 primary antibodies
  • Description

    Rabbit polyclonal to FIP200
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset, Orangutan
  • Immunogen

    Synthetic peptide within Human FIP200 aa 1544-1594 (C terminal). The exact sequence is proprietary. NP_055596.3
    Sequence:

    EGASGAS RRPWVLGKVM EKEYCQAKKA QNRFKVPLGT KFYRVKAVSW NKKV


    Database link: Q8TDY2
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa, 293T and TCMK1 whole cell lysates.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 99% Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab176816 was affinity purified using an epitope specific to FIP200 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • FAK / PYK
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • APG gene products
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Autophagosome
    • Metabolism
    • Types of disease
    • Diabetes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab176816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at 2-5 µg/mg of lysate.
WB
1/2000 - 1/10000. Predicted molecular weight: 183 kDa.
Notes
IP
Use at 2-5 µg/mg of lysate.
WB
1/2000 - 1/10000. Predicted molecular weight: 183 kDa.

Target

  • Function

    Implicated in the regulation of RB1 expression. Functions as a DNA-binding transcription factor. Is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. Required for autophagosome formation (By similarity). Inhibits PTK2/FAK1 and PTK2B/PYK2 activity and activation of downstream signaling pathways.
  • Tissue specificity

    Expression levels correlated closely with those of RB1 in cancer cell lines as well as in various normal human tissues. Abundantly expressed in human musculoskeletal and cultured osteosarcoma cells.
  • Developmental stage

    Expression was difficult to detect in immature proliferating chondroblasts or myogenic cells in embryos, but became obvious and prominent concomitantly with the maturation of osteocytes, chondrocytes, and skeletal muscle cells. Expression in these musculoskeletal cells increased with RB1 expression, which is linked to the terminal differentiation of many tissues and cells. The introduction of the wild-type protein decreased the formation of macroscopic colonies in a cell growth assay.
  • Cellular localization

    Nucleus. Cytoplasm > cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
  • Target information above from: UniProt accession Q8TDY2 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 464173 Chimpanzee
    • Entrez Gene: 100407986 Common marmoset
    • Entrez Gene: 539858 Cow
    • Entrez Gene: 477876 Dog
    • Entrez Gene: 100717631 Guinea pig
    • Entrez Gene: 100050327 Horse
    • Entrez Gene: 9821 Human
    • Entrez Gene: 12421 Mouse
    • Entrez Gene: 100172027 Orangutan
    • Entrez Gene: 100622699 Pig
    • Entrez Gene: 312927 Rat
    • Entrez Gene: 710330 Rhesus monkey
    • Omim: 606837 Human
    • SwissProt: Q8TDY2 Human
    • SwissProt: Q9ESK9 Mouse
    • Unigene: 196102 Human
    • Unigene: 293811 Mouse
    see all
  • Alternative names

    • FAK family kinase-interacting protein of 200 kDa antibody
    • FIP200 antibody
    • RB1-inducible coiled-coil protein 1 antibody
    • RB1CC1 antibody
    • RBCC1_HUMAN antibody
    see all

Images

  • Western blot - Anti-FIP200 antibody (ab176816)
    Western blot - Anti-FIP200 antibody (ab176816)
    All lanes : Anti-FIP200 antibody (ab176816) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysates at 50 µg
    Lane 2 : HeLa whole cell lysates at 15 µg
    Lane 3 : HeLa whole cell lysates at 5 µg
    Lane 4 : 293T whole cell lysates at 50 µg

    Developed using the ECL technique.

    Predicted band size: 183 kDa


    Exposure time: 30 seconds
  • Western blot - Anti-FIP200 antibody (ab176816)
    Western blot - Anti-FIP200 antibody (ab176816)
    All lanes : Anti-FIP200 antibody (ab176816) at 0.2 µg/ml

    Lane 1 : TCMK1 whole cell lysates at 50 µg
    Lane 2 : TCMK1 whole cell lysates at 15 µg

    Developed using the ECL technique.

    Predicted band size: 183 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-FIP200 antibody (ab176816)
    Immunoprecipitation - Anti-FIP200 antibody (ab176816)

    ab176816 at 0.1 µg/ml detecting FIP200 in HeLa whole cell lysate by WB following IP. 

    Lane 1: ab176816 at 3µg/mg of lysate
    Lane 2: Control IgG.

    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

    Detection: Chemiluminescence with an exposure time of 30 seconds.

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab176816? Please let us know so that we can cite the reference in this datasheet.

ab176816 has been referenced in 5 publications.

  • Xu HJ  et al. Down-regulation of exosomal microRNA-224-3p derived from bone marrow-derived mesenchymal stem cells potentiates angiogenesis in traumatic osteonecrosis of the femoral head. FASEB J N/A:fj201801618RRR (2019). PubMed: 30964699
  • Dudley LJ  et al. Intrinsic lipid binding activity of ATG16L1 supports efficient membrane anchoring and autophagy. EMBO J 38:N/A (2019). PubMed: 30936093
  • Fraser J  et al. Targeting of early endosomes by autophagy facilitates EGFR recycling and signalling. EMBO Rep 20:e47734 (2019). PubMed: 31448519
  • Shin JH  et al. Down-regulated TMED10 in Alzheimer disease induces autophagy via ATG4B activation. Autophagy 15:1495-1505 (2019). PubMed: 30821607
  • Fang W  et al. miR-224-3p inhibits autophagy in cervical cancer cells by targeting FIP200. Sci Rep 6:33229 (2016). PubMed: 27615604

Customer reviews and Q&As

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