Anti-FIP200 antibody (ab176816)
Key features and details
- Rabbit polyclonal to FIP200
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FIP200 antibody
See all FIP200 primary antibodies -
Description
Rabbit polyclonal to FIP200 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset, Orangutan
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Immunogen
Synthetic peptide within Human FIP200 aa 1544-1594 (C terminal). The exact sequence is proprietary. NP_055596.3
Sequence:EGASGAS RRPWVLGKVM EKEYCQAKKA QNRFKVPLGT KFYRVKAVSW NKKV
Database link: Q8TDY2 -
Positive control
- HeLa, 293T and TCMK1 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab176816 was affinity purified using an epitope specific to FIP200 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at 2-5 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 183 kDa.
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| Notes |
|---|
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IP
Use at 2-5 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 183 kDa. |
Target
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Function
Implicated in the regulation of RB1 expression. Functions as a DNA-binding transcription factor. Is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. Required for autophagosome formation (By similarity). Inhibits PTK2/FAK1 and PTK2B/PYK2 activity and activation of downstream signaling pathways. -
Tissue specificity
Expression levels correlated closely with those of RB1 in cancer cell lines as well as in various normal human tissues. Abundantly expressed in human musculoskeletal and cultured osteosarcoma cells. -
Developmental stage
Expression was difficult to detect in immature proliferating chondroblasts or myogenic cells in embryos, but became obvious and prominent concomitantly with the maturation of osteocytes, chondrocytes, and skeletal muscle cells. Expression in these musculoskeletal cells increased with RB1 expression, which is linked to the terminal differentiation of many tissues and cells. The introduction of the wild-type protein decreased the formation of macroscopic colonies in a cell growth assay. -
Cellular localization
Nucleus. Cytoplasm > cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome. - Information by UniProt
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Database links
- Entrez Gene: 464173 Chimpanzee
- Entrez Gene: 100407986 Common marmoset
- Entrez Gene: 539858 Cow
- Entrez Gene: 477876 Dog
- Entrez Gene: 100717631 Guinea pig
- Entrez Gene: 100050327 Horse
- Entrez Gene: 9821 Human
- Entrez Gene: 12421 Mouse
see all -
Alternative names
- FAK family kinase-interacting protein of 200 kDa antibody
- FIP200 antibody
- RB1-inducible coiled-coil protein 1 antibody
see all
Images
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Western blot - Anti-FIP200 antibody (ab176816)All lanes : Anti-FIP200 antibody (ab176816) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysates at 50 µg
Lane 2 : HeLa whole cell lysates at 15 µg
Lane 3 : HeLa whole cell lysates at 5 µg
Lane 4 : 293T whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 183 kDa
Exposure time: 30 seconds -
Western blot - Anti-FIP200 antibody (ab176816)All lanes : Anti-FIP200 antibody (ab176816) at 0.2 µg/ml
Lane 1 : TCMK1 whole cell lysates at 50 µg
Lane 2 : TCMK1 whole cell lysates at 15 µg
Developed using the ECL technique.
Predicted band size: 183 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-FIP200 antibody (ab176816)ab176816 at 0.1 µg/ml detecting FIP200 in HeLa whole cell lysate by WB following IP.
Lane 1: ab176816 at 3µg/mg of lysate
Lane 2: Control IgG.In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab176816 has been referenced in 5 publications.
- Xu HJ et al. Down-regulation of exosomal microRNA-224-3p derived from bone marrow-derived mesenchymal stem cells potentiates angiogenesis in traumatic osteonecrosis of the femoral head. FASEB J N/A:fj201801618RRR (2019). PubMed: 30964699
- Dudley LJ et al. Intrinsic lipid binding activity of ATG16L1 supports efficient membrane anchoring and autophagy. EMBO J 38:N/A (2019). PubMed: 30936093
- Fraser J et al. Targeting of early endosomes by autophagy facilitates EGFR recycling and signalling. EMBO Rep 20:e47734 (2019). PubMed: 31448519
- Shin JH et al. Down-regulated TMED10 in Alzheimer disease induces autophagy via ATG4B activation. Autophagy 15:1495-1505 (2019). PubMed: 30821607
- Fang W et al. miR-224-3p inhibits autophagy in cervical cancer cells by targeting FIP200. Sci Rep 6:33229 (2016). PubMed: 27615604