Overview

  • Product name

  • Description

    Rabbit polyclonal to FIP200
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human FIP200 (N terminal). The exact sequence is proprietary.
    Database link: Q8TDY2

  • Positive control

    • WB: HEK-293T and A431 whole cell lysate; FIP200-transfected HEK-293T whole cell lysate. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 183 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Implicated in the regulation of RB1 expression. Functions as a DNA-binding transcription factor. Is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. Required for autophagosome formation (By similarity). Inhibits PTK2/FAK1 and PTK2B/PYK2 activity and activation of downstream signaling pathways.
  • Tissue specificity

    Expression levels correlated closely with those of RB1 in cancer cell lines as well as in various normal human tissues. Abundantly expressed in human musculoskeletal and cultured osteosarcoma cells.
  • Developmental stage

    Expression was difficult to detect in immature proliferating chondroblasts or myogenic cells in embryos, but became obvious and prominent concomitantly with the maturation of osteocytes, chondrocytes, and skeletal muscle cells. Expression in these musculoskeletal cells increased with RB1 expression, which is linked to the terminal differentiation of many tissues and cells. The introduction of the wild-type protein decreased the formation of macroscopic colonies in a cell growth assay.
  • Cellular localization

    Nucleus. Cytoplasm > cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • FAK family kinase-interacting protein of 200 kDa antibody
    • FIP200 antibody
    • RB1-inducible coiled-coil protein 1 antibody
    • RB1CC1 antibody
    • RBCC1_HUMAN antibody
    see all

Images

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for FIP200 (green) using ab227726 at 1/200 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • All lanes : Anti-FIP200 antibody (ab227726) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 183 kDa



    5 % SDS-PAGE gel.

  • All lanes : Anti-FIP200 antibody (ab227726) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : FIP200-transfected HEK-293T whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 183 kDa



    5 % SDS-PAGE gel.

References

ab227726 has not yet been referenced specifically in any publications.

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