Product nameAnti-Firefly Luciferase antibody
See all Firefly Luciferase primary antibodies
DescriptionGoat polyclonal to Firefly Luciferase
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Firefly
Full length native protein (purified) corresponding to Firefly (Photinus pyralis) Firefly Luciferase aa 1-550.
MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVN ITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGV AVAPANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKII IMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSG STGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMF TTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTL IDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAIL ITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVD YVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL
Database link: P08659
- Luciferase protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
Purification notesab181640 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. ab181640 is sterile filtered.
Our Abpromise guarantee covers the use of ab181640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 61 kDa.|
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||1/1000 - 1/5000.|
RelevanceLuciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
- SwissProt: P08659 Firefly
- ec 126.96.36.199 antibody
- Firefly antibody
- Luciferase antibody
- Luciferin 4 monooxygenase antibody
All lanes : Anti-Firefly Luciferase antibody (ab181640) at 1/1000 dilution
Lane 1 : Non-transfected 293 cell lysate
Lane 2 : Firefly Luciferase transfected 293 cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : NIH3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Donkey anti-Goat IgG H&L (IRDye® 800CW) preadsorbed (ab216775) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Lanes 1 - 4: Merged signal (red and green). Green – ab181640 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab181640 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Donkey anti-Goat IgG H&L (IRDye® 800CW) preabsorbed (ab216775) and Donkey anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216778) at a 1:10000 dilution for 1hr at room temperature and then imaged.
Anti-Firefly Luciferase antibody (ab181640) at 1/1000 dilution + Luciferase protein at 0.05 µg
Peroxidase anti-goat secondary antibody at 1/40000 dilution
Predicted band size: 61 kDa
This product has been referenced in:
- Crowley C et al. Non-Invasive Longitudinal Bioluminescence Imaging of Human Mesoangioblasts in Bioengineered Esophagi. Tissue Eng Part C Methods 25:103-113 (2019). Read more (PubMed: 30648471) »
- Liu C et al. Two Arabidopsis Receptor-like Cytoplasmic Kinases SZE1 and SZE2 Associate with the ZAR1-ZED1 Complex and Are Required for Effector-Triggered Immunity. Mol Plant 12:967-983 (2019). Read more (PubMed: 30947022) »