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Dear Sir/Madam, We ordered the antibody below for immunostaining of formalin-fixed, paraffin-embedded prostate samples from transgenic mice. Luciferase activity assay showed the prostate samples from transgenic mice express luciferase. However, according to the protocol listed in Abcam, the prostate samples from both wild type (negative control) and transgenic mice have strong staining with no difference even at 1:1000 dilution of the antibody. Can we replace another luciferase antibody from Abcam?
Asked on Aug 28 2012
Thank you for contacting us.
I am sorry that this product has not performed as should be expected. This product is covered by our Abpromise guarantee. We are happy provide scientific support at any time. If you are using the product in applications listed on the datasheet and contact us within six months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue.
Would you be able to send me the details of your protocol so that I may get a better understanding of the problemms which you are facing. These details are also important should we decide that this product needs further internal testing.
Could you also provide the Abcam order confirmation number or PO used?
Thank you for your cooperation, I look forward to your response. Please do not hesitate to contact us if you need any more advice or information.
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Answered on Aug 28 2012