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I had ordered for an
anti-luciferase antibody, Ab21176, for the purpose of
immunofluorescence staining on formalin fixed slides. Unfortunately,
this has not worked for me too well. I would like to probe for my
protein of interest using Western Blot instead. Can I use this same
antibody? If yes do you have recommendations on the dilution?
Asked on Sep 21 2012
Thank you for contacting Abcam regarding ab21176.
I am sorry that you were experiencing difficulties with this antibody in IHC. If you believe this is due to the antibody being defective, I am happy to provide additional technical assistance with troubleshooting or a credit, as this product is guaranteed for IF under our Abpromise. Please provide some additional protocol information.
Regarding using the antibody in WB, we are not sure if this antibody will be suitable for western, but would recommend a starting dilution of 1 ug/ml.
I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions or concerns.
Answered on Sep 21 2012