Immunohistochemistry (Frozen sections) abreview for Anti-Firefly Luciferase antibody

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample
Mouse Tissue sections (mouse cerebellum)
Specification
mouse cerebellum
Permeabilization
Yes - PBS + 0.1% tritonx100 (PBST) for 10 min
Fixative
Acetone

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 10% goat serum in PBST
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit Alexa 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 555
Dilution
1/1000

Additional data

Additional Notes
No retrieval is required. Works upon permeabilization in PBS + 0.1% tritonx100 (PBST) for 10 minutes

Ms. Radhi Praba Velayutham

Verified customer

Submitted Oct 08 2013

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