Red histogram: luciferase+ cells (GFP+)
Black histogram: Control cells treated in same condition excepting anti-luciférase antibody (representing non-specific staining with GFP secondary antibody)
After incubation with anti-luciferase antibody, cells were washed 2 times with commercial wash buffer, then secondary antibody and DAPI were added 20min. Cells were washed 4 times with commercial wash buffer and counted on a LSRII cytometer (BD).
Dr. Francois Daubeuf
Submitted Dec 05 2016
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