Flow Cytometry abreview for Anti-Firefly Luciferase antibody

Excellent
Abreviews
Application
Flow Cytometry
Sample
Human Cell (breast cancer cell line transfected to express luc)
Permeabilization
Yes - Cells were permeabilized using commercial kit
Gating Strategy
DAPI+ cells
Specification
breast cancer cell line transfected to express luc
Preparation
Cell harvesting/tissue preparation method: Cells in culture
Sample buffer: PBS-EDTA 3mM
Fixation
Cells were fixed using commercial kit

Other product details

Incubation time
20 minute(s) · Temperature: 20°C · Diluent: Commercial wash buffer
Dilution
1/200

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein

Additional data

Additional Notes
Red histogram: luciferase+ cells (GFP+) Black histogram: Control cells treated in same condition excepting anti-luciférase antibody (representing non-specific staining with GFP secondary antibody) After incubation with anti-luciferase antibody, cells were washed 2 times with commercial wash buffer, then secondary antibody and DAPI were added 20min. Cells were washed 4 times with commercial wash buffer and counted on a LSRII cytometer (BD).

Dr. Francois Daubeuf

Verified customer

Submitted Dec 05 2016

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