Western blot abreview for Anti-Firefly Luciferase antibody

Inconclusive
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (4-12 Tris-Bis)
Loading amount
40 µg
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: IRDye 680
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye­ 680RD

Detection

Detection method
Licor
Exposure
10 second(s)
Positive control
Green channel = B-actin (42 kDa)

Additional data

Additional Notes
Too many non-specific bands inconclusive.

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Submitted May 31 2017

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