Flow Cytometry abreview for Anti-Firefly Luciferase antibody

Inconclusive
Abreviews
Application
Flow Cytometry
Sample
Human Cell (Jurkat)
Permeabilization
Yes - eBio 1x Perm Buffer Foxp3 kit, Methanol, Acetone
Gating Strategy
cells, singlets
Specification
Jurkat
Fixation
eBio Fix/Perm, 2%FA, Acetone

Other product details

Incubation time
30 minute(s) · Temperature: 4°C
Dilution
1/2500

Secondary antibody

Dilution
1/100
Name
Non-Abcam antibody was used: Brilliant Violet 480™ AffiniPure Goat Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Other

Additional data

Abcam response
This antibody was tested in flow cytometry using our Abtrial program. The results indicate that this antibody might not be suitable for flow or further optimization may be necessary.

Abcam user community

Verified customer

Submitted Mar 06 2019

Sign up