Recombinant
RabMAb

Recombinant Anti-Firefly Luciferase antibody [EPR17789] (Phycoerythrin) (ab237253)

Overview

  • Product name

    Anti-Firefly Luciferase antibody [EPR17789] (Phycoerythrin)
    See all Firefly Luciferase primary antibodies
  • Description

    Rabbit monoclonal [EPR17789] to Firefly Luciferase (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Firefly
  • Immunogen

    Synthetic peptide within Firefly Firefly Luciferase aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P08659

  • Positive control

    • Flow Cyt: HEK-293T cells transfected with GFP-tagged Firefly Luciferase.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17789
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab237253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50.

Target

  • Relevance

    Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
  • Cellular localization

    Peroxisome
  • Database links

  • Alternative names

    • ec 1.13.12.7 antibody
    • Firefly antibody
    • Luciferase antibody
    • Luciferin 4 monooxygenase antibody

Images

  • HEK-293T cells transfected with GFP-tagged Firefly Luciferase stained with ab237253 (right). The cells were fixed with 4% formaldehyde and then permeabilized with 90% methanol. The cells were then incubated in 1x PBS/ 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab237253 (right)) or Rabbit IgG (monoclonal) Phycoerythrin Isotype control (ab209478 (left)) (1x106 in 100µl at 1:50 dilution (10 µg/ml)) for 30 min at 22°C.

    Acquisition of >5,000 events were collected using a 20 mW Blue laser (488 nm) and 585/42 bandpass filter.

References

ab237253 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab237253.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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