Recombinant Anti-Firefly Luciferase antibody [EPR17790] (ab185924)
![Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)](https://www.abcam.com/ps/products/185/ab185924/Images/ab185924-243253-anti-firefly-luciferase-antibody-epr17790-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17790] to Firefly Luciferase
- Suitable for: Flow Cyt, ICC/IF, WB
- Reacts with: Firefly
Overview
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Product name
Anti-Firefly Luciferase antibody [EPR17790]
See all Firefly Luciferase primary antibodies -
Description
Rabbit monoclonal [EPR17790] to Firefly Luciferase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt FireflyICC/IF FireflyWB Firefly
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Firefly Luciferase transfected HEK293 whole cell lysate. ICC/IF: Firefly Luciferase transfected HEK293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17790 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Firefly
|
| ICC/IF |
Firefly
|
| WB |
Firefly
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/500.
|
|
| WB |
1/20000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
|
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
|
ICC/IF
1/500. |
|
WB
1/20000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
Target
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Relevance
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. -
Cellular localization
Peroxisome -
Database links
- SwissProt: P08659 Firefly
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Alternative names
- ec 1.13.12.7 antibody
- Firefly antibody
- Luciferase antibody
- Luciferin 4 monooxygenase antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293T (Human epithelial cells from embryonic kidney) cells transfected with Empty vector or Firefly Luciferase, labeling Firefly Luciferase with ab185924 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HEK293T cells transfected with Firefly Luciferase is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1: ab185924 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
![Flow Cytometry - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)](https://www.abcam.com/ps/products/185/ab185924/Images/ab185924-273206-anti-firefly-luciferase-antibody-epr17790-flow-cytometry.jpg)
Flow Cytometry - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)Flow Cytometry analysis of 293T (Human epithelial cell line from embryonic kidney) cells transfected with GFP tagged Firefly Luciferase labeled with purified ab185924 at 1/70 dilution (10µg/ml, Right panel). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 647)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Left panel) was used as the isotype control.
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![Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)](https://www.abcam.com/ps/products/185/ab185924/Images/ab185924-243248-anti-firefly-luciferase-antibody-epr17790-western-blot.jpg)
Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)All lanes : Anti-Firefly Luciferase antibody [EPR17790] (ab185924) at 1/20000 dilution
Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate
Lane 2 : Firefly Luciferase transfected HEK293 (Human embryonic kidney) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)](https://www.abcam.com/ps/products/185/ab185924/Images/ab185924-243250-anti-firefly-luciferase-antibody-epr17790-western-blot.jpg)
Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)All lanes : Anti-Firefly Luciferase antibody [EPR17790] (ab185924) at 1/1000 dilution
Lane 1 : Firefly Luciferase transfected HEK293 (human embryonic kidney) whole cell lysate at 10 µg
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg
Lane 5 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 20 µg
Lane 6 : Human fetal brain lysate at 10 µg
Lane 7 : Human fetal heart lysate at 10 µg
Lane 8 : Human fetal kidney lysate at 10 µg
Lane 9 : Human fetal spleen lysate at 10 µg
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)](https://www.abcam.com/ps/products/185/ab185924/Images/ab185924-243252-anti-firefly-luciferase-antibody-epr17790-western-blot.jpg)
Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)All lanes : Anti-Firefly Luciferase antibody [EPR17790] (ab185924) at 1/1000 dilution
Lane 1 : Firefly Luciferase transfected HEK293 (Human embryonic kidney) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse heart lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Mouse spleen lysate
Lane 6 : Rat brain lysate
Lane 7 : Rat heart lysate
Lane 8 : Rat kidney lysate
Lane 9 : Rat spleen lysate
Lane 10 : C6 (Rat glial tumor cells) whole cell lysate
Lane 11 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 12 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 13 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-Firefly Luciferase antibody [EPR17790] (ab185924)](https://www.abcam.com/ps/products/185/ab185924/Images/ab185924-6-benefits-of-recombinant-antibodies.png)
Anti-Firefly Luciferase antibody [EPR17790] (ab185924)
Protocols
Datasheets and documents
Certificate of Compliance
References (4)
ab185924 has been referenced in 4 publications.
- Ryu HG et al. Upf1 regulates neurite outgrowth and branching by transcriptional and post-transcriptional modulation of Arc. J Cell Sci 132:N/A (2019). PubMed: 30584064
- Benhabiles H et al. Optimized approach for the identification of highly efficient correctors of nonsense mutations in human diseases. PLoS One 12:e0187930 (2017). PubMed: 29131862
- Li D et al. An Ebola Virus-Like Particle-Based Reporter System Enables Evaluation of Antiviral Drugs In Vivo under Non-Biosafety Level 4 Conditions. J Virol 90:8720-8 (2016). PubMed: 27440895
- Simonyan H et al. A synthetic luciferin improves in vivo bioluminescence imaging of gene expression in cardiovascular brain regions. Physiol Genomics 48:762-770 (2016). PubMed: 27614203
