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    firefly-luciferase-antibody-epr17790-ab185924.pdf

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Tags & Cell Markers Fusion / Marker Proteins Luciferase
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RecombinantRabMAb

Recombinant Anti-Firefly Luciferase antibody [EPR17790] (ab185924)

  • Datasheet
  • Certificate of Compliance
Reviews (2) Submit a question References (4)

Product price, shipping and contact information

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Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)
  • Flow Cytometry - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)
  • Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)
  • Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)
  • Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)
  • Anti-Firefly Luciferase antibody [EPR17790] (ab185924)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17790] to Firefly Luciferase
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Reacts with: Firefly

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Overview

  • Product name

    Anti-Firefly Luciferase antibody [EPR17790]
    See all Firefly Luciferase primary antibodies
  • Description

    Rabbit monoclonal [EPR17790] to Firefly Luciferase
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Firefly
    ICC/IF
    Firefly
    WB
    Firefly
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Firefly Luciferase transfected HEK293 whole cell lysate. ICC/IF: Firefly Luciferase transfected HEK293T cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17790
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • Luciferase

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Firefly Luciferase antibody [EPR17790] (ab214950)
    • Anti-Firefly Luciferase antibody [EPR17790] - BSA and Azide free (ab232629)
    • Alexa Fluor® 647 Anti-Firefly Luciferase antibody [EPR17790] (ab233049)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Firefly Luciferase protein (ab100961)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Firefly
ICC/IF
Firefly
WB
Firefly
Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
1/500.
WB
1/20000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
Notes
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
1/500.
WB
1/20000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).

Target

  • Relevance

    Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
  • Cellular localization

    Peroxisome
  • Database links

    • SwissProt: P08659 Firefly
    • Alternative names

      • ec 1.13.12.7 antibody
      • Firefly antibody
      • Luciferase antibody
      • Luciferin 4 monooxygenase antibody

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)
      Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293T (Human epithelial cells from embryonic kidney) cells transfected with Empty vector or Firefly Luciferase, labeling Firefly Luciferase with ab185924 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HEK293T cells transfected with Firefly Luciferase is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows;
      -ve control 1: ab185924 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    • Flow Cytometry - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)
      Flow Cytometry - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)

      Flow Cytometry analysis of 293T (Human epithelial cell line from embryonic kidney) cells transfected with GFP tagged Firefly Luciferase labeled with purified ab185924 at 1/70 dilution (10µg/ml, Right panel). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 647)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Left panel) was used as the isotype control.

    • Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)
      Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)
      All lanes : Anti-Firefly Luciferase antibody [EPR17790] (ab185924) at 1/20000 dilution

      Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate
      Lane 2 : Firefly Luciferase transfected HEK293 (Human embryonic kidney) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 61 kDa
      Observed band size: 61 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)
      Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)
      All lanes : Anti-Firefly Luciferase antibody [EPR17790] (ab185924) at 1/1000 dilution

      Lane 1 : Firefly Luciferase transfected HEK293 (human embryonic kidney) whole cell lysate at 10 µg
      Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg
      Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
      Lane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg
      Lane 5 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 20 µg
      Lane 6 : Human fetal brain lysate at 10 µg
      Lane 7 : Human fetal heart lysate at 10 µg
      Lane 8 : Human fetal kidney lysate at 10 µg
      Lane 9 : Human fetal spleen lysate at 10 µg

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 61 kDa
      Observed band size: 61 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)
      Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)
      All lanes : Anti-Firefly Luciferase antibody [EPR17790] (ab185924) at 1/1000 dilution

      Lane 1 : Firefly Luciferase transfected HEK293 (Human embryonic kidney) whole cell lysate
      Lane 2 : Mouse brain lysate
      Lane 3 : Mouse heart lysate
      Lane 4 : Mouse kidney lysate
      Lane 5 : Mouse spleen lysate
      Lane 6 : Rat brain lysate
      Lane 7 : Rat heart lysate
      Lane 8 : Rat kidney lysate
      Lane 9 : Rat spleen lysate
      Lane 10 : C6 (Rat glial tumor cells) whole cell lysate
      Lane 11 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
      Lane 12 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
      Lane 13 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 61 kDa
      Observed band size: 61 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Anti-Firefly Luciferase antibody [EPR17790] (ab185924)
      Anti-Firefly Luciferase antibody [EPR17790] (ab185924)

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (4)

    Publishing research using ab185924? Please let us know so that we can cite the reference in this datasheet.

    ab185924 has been referenced in 4 publications.

    • Ryu HG  et al. Upf1 regulates neurite outgrowth and branching by transcriptional and post-transcriptional modulation of Arc. J Cell Sci 132:N/A (2019). PubMed: 30584064
    • Benhabiles H  et al. Optimized approach for the identification of highly efficient correctors of nonsense mutations in human diseases. PLoS One 12:e0187930 (2017). PubMed: 29131862
    • Li D  et al. An Ebola Virus-Like Particle-Based Reporter System Enables Evaluation of Antiviral Drugs In Vivo under Non-Biosafety Level 4 Conditions. J Virol 90:8720-8 (2016). PubMed: 27440895
    • Simonyan H  et al. A synthetic luciferin improves in vivo bioluminescence imaging of gene expression in cardiovascular brain regions. Physiol Genomics 48:762-770 (2016). PubMed: 27614203

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Firefly Luciferase antibody [EPR17790]

    Below Average
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Pancreas and Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Permeabilization
    No
    Specification
    Pancreas and Liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 12 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Firefly Luciferase antibody [EPR17790]

    Inconclusive
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Pancreas)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate (10mM)
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Pancreas
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 14 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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