Recombinant
RabMAb

Recombinant Anti-Firefly Luciferase antibody [EPR17790] (Alexa Fluor® 488) (ab214950)

Rabbit recombinant monoclonal Firefly Luciferase antibody [EPR17790] conjugated to Alexa Fluor® 488. Validated in ICC/IF and tested in Firefly.

Overview

  • Product name

    Anti-Firefly Luciferase antibody [EPR17790] (Alexa Fluor® 488)
    See all Firefly Luciferase primary antibodies
  • Description

    Rabbit monoclonal [EPR17790] to Firefly Luciferase (Alexa Fluor® 488)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Firefly
  • Immunogen

    Synthetic peptide within Firefly Firefly Luciferase aa 150-250. The exact sequence is proprietary.
    Database link: P08659

  • Positive control

    • ICC/IF: HEK293T cells transfected with RFP-tagged Firefly Luciferase
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 30% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17790
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab214950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.

This product gave a positive signal in HEK293T cells transfected with RFP-tagged Firefly Luciferase fixed with 4% formaldehyde (10 min)

Target

  • Relevance

    Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
  • Cellular localization

    Peroxisome
  • Database links

  • Alternative names

    • ec 1.13.12.7 antibody
    • Firefly antibody
    • Luciferase antibody
    • Luciferin 4 monooxygenase antibody

Images

  • ab214950 staining Firefly Luciferase in HEK293T cells transfected with RFP-tagged Firefly Luciferase expression construct or RFP-only-tagged expression construct. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.

    The cells were then incubated overnight at +4°C with ab214950 at 1/200 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue) and RFP is shown in red.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

This product has been referenced in:

  • Mosaad EO  et al. Constraints to counting bioluminescence producing cells by a commonly used transgene promoter and its implications for experimental design. Sci Rep 9:11334 (2019). Read more (PubMed: 31383876) »
See 1 Publication for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (WNT3A protein treated BMSCs)
Permeabilization
Yes - 0.5% Triton
Specification
WNT3A protein treated BMSCs
Blocking step
R.T.U. Animal-Free Block and Diluent as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 23°C
Fixative
Formaldehyde

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Verified customer

Submitted Oct 01 2019

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