Recombinant Anti-Firefly Luciferase antibody [LAS126A] (ab241541)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [LAS126A] to Firefly Luciferase
- Suitable for: WB
- Reacts with: Firefly
Related conjugates and formulations
Overview
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Product name
Anti-Firefly Luciferase antibody [LAS126A]
See all Firefly Luciferase primary antibodies -
Description
Rat monoclonal [LAS126A] to Firefly Luciferase -
Host species
Rat -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Firefly -
Immunogen
Recombinant full length protein. This information is considered to be commercially sensitive.
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Positive control
- WB: Firefly Luciferase transfected HEK293 (Human embryonic kidney) whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
LAS126A -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab241541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.115 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
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Notes |
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WB
Use a concentration of 0.115 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
Target
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Relevance
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. -
Cellular localization
Peroxisome -
Database links
- SwissProt: P08659 Firefly
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Alternative names
- ec 1.13.12.7 antibody
- Firefly antibody
- Luciferase antibody
- Luciferin 4 monooxygenase antibody
Images
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All lanes : Anti-Firefly Luciferase antibody [LAS126A] (ab241541) at 1/5000 dilution
Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate
Lane 2 : Firefly Luciferase transfected HEK293 (Human embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 1 secondBlocking and dilution buffer: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab241541 has not yet been referenced specifically in any publications.