Recombinant

Recombinant Anti-Firefly Luciferase antibody [LAS126A] (ab241541)

Overview

  • Product name

    Anti-Firefly Luciferase antibody [LAS126A]
    See all Firefly Luciferase primary antibodies
  • Description

    Rat monoclonal [LAS126A] to Firefly Luciferase
  • Host species

    Rat
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Firefly
  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Firefly Luciferase transfected HEK293 (Human embryonic kidney) whole cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    LAS126A
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Relevance

      Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
    • Cellular localization

      Peroxisome
    • Database links

    • Alternative names

      • ec 1.13.12.7 antibody
      • Firefly antibody
      • Luciferase antibody
      • Luciferin 4 monooxygenase antibody

    Images

    • All lanes : Anti-Firefly Luciferase antibody [LAS126A] (ab241541) at 1/5000 dilution

      Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate
      Lane 2 : Firefly Luciferase transfected HEK293 (Human embryonic kidney) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/20000 dilution

      Predicted band size: 61 kDa
      Observed band size: 61 kDa


      Exposure time: 1 second


      Blocking and dilution buffer: 5% NFDM/TBST.

    References

    ab241541 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab241541.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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