Product nameAnti-Firefly Luciferase antibody [LAS126A] - BSA and Azide free
See all Firefly Luciferase primary antibodies
DescriptionRat monoclonal [LAS126A] to Firefly Luciferase - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Firefly
Recombinant full length protein. This information is considered to be commercially sensitive.
- WB: Firefly Luciferase transfected HEK293 (Human embryonic kidney) whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab252791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).|
RelevanceLuciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
- SwissProt: P08659 Firefly
- ec 126.96.36.199 antibody
- Firefly antibody
- Luciferase antibody
- Luciferin 4 monooxygenase antibody
All lanes : Anti-Firefly Luciferase antibody [LAS126A] (ab241541) at 1/5000 dilution
Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate
Lane 2 : Firefly Luciferase transfected HEK293 (Human embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 1 second
Blocking and dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab241541).
ab252791 has not yet been referenced specifically in any publications.