Recombinant Anti-Firefly Luciferase antibody [LAS126A] - BSA and Azide free (ab252791)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [LAS126A] to Firefly Luciferase - BSA and Azide free
- Suitable for: WB
- Reacts with: Firefly
Related conjugates and formulations
Overview
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Product name
Anti-Firefly Luciferase antibody [LAS126A] - BSA and Azide free
See all Firefly Luciferase primary antibodies -
Description
Rat monoclonal [LAS126A] to Firefly Luciferase - BSA and Azide free -
Host species
Rat -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Firefly -
Immunogen
Recombinant full length protein. This information is considered to be commercially sensitive.
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Positive control
- WB: Firefly Luciferase transfected HEK293 (Human embryonic kidney) whole cell lysate.
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General notes
ab252791 is the carrier-free version of ab241541.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
LAS126A -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.115 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
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Notes |
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WB
Use a concentration of 0.115 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
Target
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Relevance
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. -
Cellular localization
Peroxisome -
Database links
- SwissProt: P08659 Firefly
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Alternative names
- ec 1.13.12.7 antibody
- Firefly antibody
- Luciferase antibody
- Luciferin 4 monooxygenase antibody
Images
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All lanes : Anti-Firefly Luciferase antibody [LAS126A] (ab241541) at 1/5000 dilution
Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate
Lane 2 : Firefly Luciferase transfected HEK293 (Human embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 1 secondBlocking and dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab241541).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab252791 has not yet been referenced specifically in any publications.