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Hi, I have a question on your antibody product, Ab16466, a monoclonal antibody for firefly luciferase. You mention that it is " a Hybridoma produced by the fusion of spleenocytes from mice immunized with luciferase protein isolated from Photinus pyralis." Can I confirm that this antibody was produced by immunization using the whole, native protein, as opposed to a shorter peptide epitope? Can I also ask if you've identified the epitope in firefly luciferase that the antibody reacts with? Also, have you ever tested to see if the antibody inhibits the enzymatic activity of firefly luciferase?
Asked on Feb 06 2012
Thank you for contacting Abcam.
This product was created using the whole purified protein and an immunogen. The fusion of spleenocytes is an antibody production technique for creating monoclonals.
The epitope for this antibody has not yet been mapped.
To our knowledge this product has not been tested for inhibition of enzymatic activity.
I hope this information is helpful. Please let me know if you have any questions.
Answered on Feb 06 2012