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I need to find out if you have anti-firefly antibody for IP and do a western subsequently. If yes can you send me a protocol for IP with anti-firefly antibody.
Asked on Jun 19 2012
Thank you for your enquiry.
We do have a few Firefly antibodies in our catalogue (see attached file)but unfortunately, none of them have been tested in IP application. These products however can be used in WBand might also work in IP.For your information, one of our best selling antibodies is ab16466 Mouse monoclonal [Luci17] to Firefly Luciferase.
Please rest assured that all tested applications are specified on Abcam product datasheets, and these are updated as soon as any new information is brought to our attention. Although IP is not stated on the datasheet, this does not mean that the antibody will not work, just that we can not guarantee that you will get good results. Please rest assured that if we have tested it in IP and found that it does not work, the application will be stated on the datasheet as not suitable for the product.
Regarding ourrecommended IP protocol, please see the following link for more information.
I hope this information will still be helpful.If there is anything else that I can help you with please do not hesitate to contact me.
Have a nice day!
Answered on Jun 19 2012