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I have purchased an antibody (ab16466) which is 1mg/ml and i have to make 400µg/ml, can you tell me what buffer do i need to dilute it.
Asked on Jul 25 2012
Thank you for your enquiry.
Usually the buffer to use for dilutionis the same as your blocking buffer. There are some common buffers that are used which are1-3% BSA buffer, secondary antibody host species serum, Blockase, or even just PBST only.
Please check the protocols on our website for more details.
I hope this information will be helpful. If there is anything else that I can help you with please do not hesitate to contact me.
Have a nice day!
Answered on Jul 25 2012